Mirbehbahani Fatemeh Sadat, Hejazi Fatemeh, Najmoddin Najmeh, Asefnejad Azadeh
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Advanced Technologies, Shiraz University, Shiraz, Iran.
Prog Biomater. 2020 Sep;9(3):139-151. doi: 10.1007/s40204-020-00138-z. Epub 2020 Sep 28.
Artemisia annua L. has been utilized for the first time in a nanofibrous wound dressing composition. The extract of this valuable plant provides anti-inflammatory, anti-bacterial and anti-microbial properties which can be considered as a promising medicinal component in therapeutic applications. In the present work, Artemisia annua L. was picked up from Gorgan forest area of Northern Iran and its extract was prepared by methanol as the extraction solvent. In the fabrication of wound dressing, Artemisia annua L. extract was mixed with gelatin and a nanofibrous structure was formed by electrospinning technique. To have a wound dressing with acceptable stability and optimum mechanical properties, this biologically active layer was formed on a PCL nanofibrous base layer. The fabricated double-layer wound dressing was analyzed chemically, structurally, mechanically and biologically. ATR-FTIR spectra of the prepared wound dressing contain functional groups of Artemisia annua L. as peroxide groups, etc. SEM micrographs of electrospun gelatin/Artemisia annua L. confirmed the successful electrospinning process for producing Artemisia annua L.-containing nanofibers with mean diameter of 242.00 ± 67.53 nm. In vitro Artemisia annua L. release study of the fabricated wound dressings suggests a sustain release over 7 days for the crosslinked sample. In addition, evaluation of the in vitro structural stability of the prepared wound dressings confirmed the stability of the crosslinked nanofibrous structures in PBS solution environment. Biological study of the Artemisia annua L.-containing wound dressing revealed no cytotoxicity, good proliferation and attachment of the seeded fibroblasts cells and acceptable antibacterial property against Staphylococcus aureus bacteria.
青蒿首次被用于一种纳米纤维伤口敷料组合物中。这种珍贵植物的提取物具有抗炎、抗菌和抗微生物特性，可被视为治疗应用中一种有前景的药用成分。在本研究中，青蒿采自伊朗北部戈尔甘森林地区，其提取物用甲醇作为提取溶剂制备。在伤口敷料的制备过程中，将青蒿提取物与明胶混合，并通过静电纺丝技术形成纳米纤维结构。为了获得具有可接受稳定性和最佳机械性能的伤口敷料，在聚己内酯纳米纤维基层上形成了这种生物活性层。对制备的双层伤口敷料进行了化学、结构、机械和生物学分析。所制备伤口敷料的衰减全反射傅里叶变换红外光谱包含青蒿的官能团，如过氧化物基团等。静电纺丝明胶/青蒿的扫描电子显微镜照片证实了成功的静电纺丝过程，可生产出平均直径为242.00±67.53纳米的含青蒿纳米纤维。对所制备伤口敷料的体外青蒿释放研究表明，交联样品在7天内持续释放。此外，对所制备伤口敷料的体外结构稳定性评估证实了交联纳米纤维结构在磷酸盐缓冲盐溶液环境中的稳定性。对含青蒿伤口敷料的生物学研究表明，其无细胞毒性，接种的成纤维细胞具有良好的增殖和附着能力，并且对金黄色葡萄球菌具有可接受的抗菌性能。
