Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu, India.
Department of Biotechnology, PSG College of Arts and Science, Coimbatore, Tamil Nadu, India.
IET Nanobiotechnol. 2020 Oct;14(8):714-721. doi: 10.1049/iet-nbt.2020.0056.
In this present study, a hybrid Chi-FeO was prepared, characterised and evaluated for its antibacterial and antibiofilm potential against and bacterial pathogens. Intense peak around 260 nm in the ultraviolet-visible spectrum specify the formation of magnetite nanoparticles. Spherical-shaped particles with less agglomeration and particle size distribution of 3.78-46.40 nm were observed using transmission electron microscopy analysis and strong interaction of chitosan with the surface of magnetite nanoparticles was studied using field emission scanning microscopy (FESEM). X-ray diffraction analysis exhibited the polycrystalline and spinel structure configuration of the nanocomposite. Presence of Fe and O, C and Cl elements were confirmed using energy dispersive X-ray microanalysis. Fourier transform infrared spectroscopic analysis showed the reduction and formation of Chi-FeO nanocomposite. The antibacterial activity by deformation of the bacterial cell walls on treatment with Chi-FeO nanocomposite and its interaction was visualised using FESEM and the antibiofilm activity was determined using antibiofilm assay. In conclusion, this present study shows the green synthesis of Chi-FeO nanocomposite and evaluation of its antibacterial and antibiofilm potential, proving its significance in medical and biological applications.
在本研究中，制备了一种混合的 Chi-FeO，对其进行了特征描述，并评估了其对 和 细菌病原体的抗菌和抗生物膜潜力。紫外可见光谱中 260nm 左右的强峰表明了磁铁矿纳米粒子的形成。通过透射电子显微镜分析观察到具有较少团聚和粒径分布在 3.78-46.40nm 的球形颗粒，用场发射扫描显微镜（FESEM）研究了壳聚糖与磁铁矿纳米粒子表面的强相互作用。X 射线衍射分析显示了纳米复合材料的多晶和尖晶石结构构型。使用能量色散 X 射线微分析证实了 Fe 和 O、C 和 Cl 元素的存在。傅里叶变换红外光谱分析表明 Chi-FeO 纳米复合材料的还原和形成。通过 FESEM 观察 Chi-FeO 纳米复合材料处理对细菌细胞壁的变形及其相互作用，可视化其抗菌活性，并通过抗生物膜测定法确定其抗生物膜活性。总之，本研究表明 Chi-FeO 纳米复合材料的绿色合成及其抗菌和抗生物膜潜力的评估，证明了其在医学和生物学应用中的重要意义。
