Brain Research Institute Monash Sunway (BRIMS), Jeffrey Cheah School of Medicine and Health Sciences, Monash University, Jalan Lagoon Selatan, Bandar Sunway, 47500, Subang Jaya, Selangor Darul Ehsan, Malaysia.
Faculty of Science and Engineering, University of Nottingham, Broga Road, 43500, Semenyih, Selangor Darul Ehsan, Malaysia.
Appl Biochem Biotechnol. 2021 Apr;193(4):1170-1186. doi: 10.1007/s12010-020-03469-6. Epub 2020 Nov 17.
Microbial fuel cell (MFC) is a promising technology that utilizes exoelectrogens cultivated in the form of biofilm to generate power from various types of sources supplied. A metal-reducing pathway is utilized by these organisms to transfer electrons obtained from the metabolism of substrate from anaerobic respiration extracellularly. A widely established model organism that is capable of extracellular electron transfer (EET) is Shewanella oneidensis. This review highlights the strategies used in the transformation of S. oneidensis and the recent development of MFC in terms of intervention through genetic modifications. S. oneidensis was genetically engineered for several aims including the study on the underlying mechanisms of EET, and the enhancement of power generation and wastewater treating potential when used in an MFC. Through engineering S. oneidensis, genes responsible for EET are identified and strategies on enhancing the EET efficiency are studied. Overexpressing genes related to EET to enhance biofilm formation, mediator biosynthesis, and respiration appears as one of the common approaches.
微生物燃料电池（MFC）是一种很有前途的技术，它利用以生物膜形式培养的异化电子菌从各种类型的供应源中产生电能。这些生物体利用一种金属还原途径将从基质代谢中获得的电子从厌氧呼吸中转移到细胞外。能够进行细胞外电子转移（EET）的一个广泛确立的模式生物是希瓦氏菌属 oneidensis。本综述重点介绍了用于转化 S. oneidensis 的策略以及通过遗传修饰对 MFC 的最新发展。为了几个目的对 S. oneidensis 进行了基因工程改造，包括研究 EET 的潜在机制，以及增强其在 MFC 中的发电和废水处理潜力。通过工程化 S. oneidensis，可以鉴定出负责 EET 的基因，并研究提高 EET 效率的策略。过表达与 EET 增强生物膜形成、介体生物合成和呼吸相关的基因是常见的方法之一。
