Sowden J A, Roberts-Thomson P J, Zola H
Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, S.A., Australia.
Rheumatol Int. 1987;7(6):255-9. doi: 10.1007/BF00270525.
CD5 (OKT1, Leu-1) is an antigen originally associated only with T cells. This antigen has recently been detected on a population of B cells that have been implicated in autoimmune diseases, particularly in rheumatoid arthritis (RA). We determined the percentage of these cells in the peripheral blood (PB) and synovial fluid (SF) of patients with RA and other joint diseases (OJD) using flow cytometry and two-colour fluorescence. No significant difference was observed between the percentages of CD5-positive B cells in the PB of these two patient groups or healthy individuals. In comparison with PB, significantly higher percentages of these cells were observed in the SF of patients with RA, but not in SF of OJD patients. Higher percentages of B cells were also noted in RA SF. However, when B-cell percentages were accounted for, no significant difference was observed between the numbers of B cells expressing CD5 in SF. No correlation was observed between the percentages of CD5-positive B cells and the levels of rheumatoid factor (RF) or C-reactive protein (CRP). We conclude that CD5-positive B cells are an integral part of the B-cell pool of PB and that there is increased representation of B cells in SF. The increased percentages of B cells in RA SF, compared with OJD, may reflect the autoimmune phenomenon occurring in the rheumatoid joint.
CD5（OKT1，Leu-1）是一种最初仅与T细胞相关的抗原。最近在一群与自身免疫性疾病，特别是类风湿性关节炎（RA）有关的B细胞上检测到了这种抗原。我们使用流式细胞术和双色荧光法测定了RA患者以及其他关节疾病（OJD）患者外周血（PB）和滑液（SF）中这些细胞的百分比。在这两组患者的外周血以及健康个体中，CD5阳性B细胞的百分比没有显著差异。与外周血相比，RA患者滑液中这些细胞的百分比显著更高，但OJD患者的滑液中则不然。在RA滑液中也发现B细胞的百分比更高。然而，当考虑B细胞百分比时，滑液中表达CD5的B细胞数量没有显著差异。CD5阳性B细胞的百分比与类风湿因子（RF）或C反应蛋白（CRP）的水平之间没有相关性。我们得出结论，CD5阳性B细胞是外周血B细胞库的一个组成部分，并且滑液中的B细胞数量有所增加。与OJD相比，RA滑液中B细胞百分比的增加可能反映了类风湿关节中发生的自身免疫现象。
