Current Prevalence of Oral among Japanese Adults Determined Using a Nested Polymerase Chain Reaction Assay.

In Japan, gastric infection prevalence has markedly decreased with socioeconomic development. We aimed to investigate the prevalence of oral in Japanese adults in 2020 by sex, age, sampling site, and medical history. Unstimulated saliva, supragingival biofilm, and tongue coating were obtained from 88 subjects-with no complaints of upper digestive symptoms-attending a dentist's office for dental check-up or disorders. Supragingival biofilm was collected from the upper incisors, lower incisors, upper right molars and lower left molars to analyze the characteristic distribution. Oral was detected using nested polymerase chain reaction. Oral prevalence did not statistically differ by sex or age. Supragingival biofilm (30.7%) was the most common oral niche; it was also detected in 4.5% of saliva and 2.3% of tongue samples. The lower incisor was the most common site among the supragingival biofilm samples, followed by the upper incisors, lower left molars, and upper right molars. Oral DNA was frequently detected in patients with a history of gastric infection. Oral has a characteristic distribution independent of sex and age, suggesting that it is part of the normal microflora in the adult oral cavity.