Nada Essam A, Muhammad Eman M S, Ahmed Sheren F M, Tamam Asmaa M S, Abdelhamed Amr
Dermatology, Venereology and Andrology Department, Faculty of Medicine, Sohag University, Sohag, Egypt.
Pathology Department, Faculty of Medicine, Sohag University, Sohag, Egypt.
Clin Cosmet Investig Dermatol. 2020 Dec 22;13:1005-1013. doi: 10.2147/CCID.S284300. eCollection 2020.
Psoriasis vulgaris, one of the most prevalent chronic inflammatory skin diseases, is associated with metabolic syndrome (MetS). Autophagy, an intracellular degradation system is essential for cell survival and differentiation, and its dysfunction may contribute to metabolic diseases. A cross-sectional study was conducted on 38 psoriasis vulgaris patients and 16 healthy control subjects to 1) Assess immunohistochemical (IHC) expression of microtubule-associated protein light chain 3 (LC3); 2) Evaluate the relationship between Psoriasis Area Severity Index (PASI) score, and LC3 expression.
PASI score was evaluated for all psoriasis patients. Lipid profile, blood sugar, and CRP were done for all patients and controls. A punch biopsy was taken from lesional and perilesional skin of psoriasis patients and normal skin of the controls. Tissue sections were prepared. IHC LC3 staining was done and evaluated.
LC3 was nearly absent, in the epidermis of the lesional skin of psoriasis while it was strong among control (p=0.001). LC3 expression in the lesional skin of psoriasis vulgaris was lower than its expression in perilesional (p=0.001). However, LC3 expression was not significantly changed with PASI or the presence/absence of MetS.
A potential link between psoriasis vulgaris and autophagy as assessed by LC3 could be present. LC3 was down-regulated in psoriasis lesions than in normal skin. However, its expression did not change with PASI or MetS. An autophagy enhancer might be used as a possible therapeutic target in psoriasis vulgaris patients.
寻常型银屑病是最常见的慢性炎症性皮肤病之一，与代谢综合征（MetS）相关。自噬是一种细胞内降解系统，对细胞存活和分化至关重要，其功能障碍可能导致代谢性疾病。对38例寻常型银屑病患者和16例健康对照者进行了一项横断面研究，以：1）评估微管相关蛋白轻链3（LC3）的免疫组织化学（IHC）表达；2）评估银屑病面积严重程度指数（PASI）评分与LC3表达之间的关系。
对所有银屑病患者评估PASI评分。对所有患者和对照者进行血脂、血糖和CRP检测。从银屑病患者的皮损和皮损周边皮肤以及对照者的正常皮肤取打孔活检组织。制备组织切片。进行IHC LC3染色并评估。
银屑病皮损表皮中几乎不存在LC3，而在对照组中LC3表达较强（p = 0.001）。寻常型银屑病皮损中的LC3表达低于皮损周边（p = 0.001）。然而，LC3表达并未随PASI或MetS的有无而发生显著变化。
通过LC3评估，寻常型银屑病与自噬之间可能存在潜在联系。银屑病皮损中的LC3表达低于正常皮肤。然而，其表达并未随PASI或MetS而改变。自噬增强剂可能作为寻常型银屑病患者的一种潜在治疗靶点。
