Yu Jingrong, Fang Cheng, Zhang Ziyue, Zhang Guifang, Shi Lihong, Qian Jiayi, Xiong Jianping
Department of Oncology, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province 330003, People's Republic of China.
Department of Oncology, Nanchang 334 Hospital, Nanchang, Jiangxi Province, People's Republic of China.
Cancer Manag Res. 2020 Dec 21;12:13033-13042. doi: 10.2147/CMAR.S267357. eCollection 2020.
The aim of this paper was to investigate expression in gastric cancer (GC) and its effects on the biological behavior of gastric cancer cells (GCCs), and at exploring its potential mechanism.
expression in the patients' tissues and serum was detected, and the correlation of the expression with the patients' pathological data and survival rate was analyzed. Overexpression or inhibitory vectors of , () and were constructed and transfected into GCCs to observe their effects on the cells' proliferation, invasion and apoptosis.
rose in GC and was higher in GC patients with a tumor size ≥5 cm, high stages (III+IV) and lymph node metastasis. High H19 expression was associated with the poorer survival rate of the patients, so serum had a certain diagnostic value for GC. knockdown could inhibit GCCs to proliferate and invade and induce their apoptosis. can be used as the target gene of , and can be negatively regulated by this miR, so knockdown or upregulation can weaken GCCs' biological behavior changes that were caused by H19 knockdown.
can be used as a biological indicator for diagnosing GC and predicting patients' poor prognosis. Additionally, it promotes GCCs to proliferate and invade through / axis.
本文旨在研究 在胃癌（GC）中的表达及其对胃癌细胞（GCCs）生物学行为的影响，并探索其潜在机制。
检测患者组织和血清中的 表达，并分析该表达与患者病理数据及生存率的相关性。构建 、 （）和 的过表达或抑制载体并转染至GCCs中，以观察它们对细胞增殖、侵袭和凋亡的影响。
在GC中升高，在肿瘤大小≥5 cm、高分期（III + IV期）和有淋巴结转移的GC患者中更高。高H19表达与患者较差的生存率相关，因此血清 对GC有一定的诊断价值。 敲低可抑制GCCs增殖和侵袭并诱导其凋亡。 可作为 的靶基因，且该miR可对 进行负调控，因此 敲低或 上调可减弱由H19敲低引起的GCCs生物学行为变化。
可作为诊断GC和预测患者预后不良的生物学指标。此外，它通过 / 轴促进GCCs增殖和侵袭。
