Leibniz Institute of Polymer Research Dresden, Saxony 01069, Germany.
Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, QLD 4059, Australia.
STAR Protoc. 2020 Nov 21;1(3):100180. doi: 10.1016/j.xpro.2020.100180. eCollection 2020 Dec 18.
Optical slice microscopy is commonly used to characterize the morphometric features of 3D cellular cultures, such as vascularization. However, the quantitative analysis of those structures is often performed on a single 2D maximum intensity projection image, limiting the accuracy of data obtained from 3D cultures. Here, we present a protocol for the quantitative analysis of z stack images, utilizing Fiji, Amira, and WinFiber3D. This protocol facilitates the in-depth examination of vascular-like structures within 3D cell culture models. For complete details on the use and execution of this protocol, please refer to Koch et al. (2020).
光学切片显微镜常用于描述 3D 细胞培养物的形态特征，如血管生成。然而，这些结构的定量分析通常是在单个 2D 最大强度投影图像上进行的，这限制了从 3D 培养物中获得的数据的准确性。在这里，我们提出了一种利用 Fiji、Amira 和 WinFiber3D 对 z 堆叠图像进行定量分析的方案。该方案有助于深入研究 3D 细胞培养模型中的类血管结构。有关此方案的使用和执行的完整详细信息，请参见 Koch 等人（2020 年）。
