Centre for Food-borne, Environmental and Zoonotic Infectious Diseases, Public Health Agency of Canada, 370 Speedvale Avenue West, Suite #201, Guelph, Ontario N1H 7M7 (Agunos, Léger, Desruisseau, Pollari, Reid-Smith); Saskatoon, Saskatchewan (Gow, Flockhart); Saint-Hyacinthe, Quebec (Daignault); British Columbia Ministry of Agriculture, Animal Health Center, Abbotsford, British Columbia V3G 2M3 (Zabek).
Can J Vet Res. 2021 Jan;85(1):27-35.
Eggs are important to the diet of Canadians. This product is one of the supply-managed commodities in Canada, but unlike other commodities, where food safety risks are extensively explored and reported, information on the prevalence of enteric organisms (e.g., ) and antimicrobial resistance (AMR) in layers in Canada are limited. This study was conducted to determine the prevalence of select bacteria and the associated AMR patterns in layer flocks using 2 sample matrices. Farms were located within FoodNet Canada and the Canadian Integrated Program for Antimicrobial Resistance Surveillance sentinel sites (SS). Fecal samples (Ontario: ON, ON) and environmental sponge swabs (British Columbia: BC) were collected. prevalence was 29% and 8% in ON and ON, respectively, and 7% in BC. . Kentucky and . Livingstone were the most frequently isolated serovars and no . Enteritidis was detected. was not detected in the BC sponge swabs but was isolated from 89% and 53% of Ontario fecal samples (ON and ON, respectively). Seven from Ontario were ciprofloxacin-resistant. prevalence was high in both sample types (98%). Overall, tetracycline resistance among ranged from 26% to 69%. Resistance to ceftiofur ( = 2 isolates) and gentamicin ( = 2) was relatively low. There were diverse resistance patterns (excludes susceptible isolates) observed among in Ontario (10 patterns) and British Columbia (14 patterns). This study revealed that fecal samples are more informative for farm-level monitoring of pathogen and AMR prevalence. Without further validation, sponge swabs are limited in their utility for detection and thus, for public health surveillance.
鸡蛋是加拿大人饮食中重要的一部分。这种产品是加拿大受管理供应商品之一，但与其他商品不同，食品安全风险在其他商品中得到了广泛的探讨和报告，而关于加拿大蛋鸡中肠杆菌（例如）和抗生素耐药性（AMR）的流行情况的信息有限。本研究旨在使用两种样本基质来确定选择细菌的流行情况以及与蛋鸡相关的 AMR 模式。这些农场位于加拿大食品网和加拿大综合抗生素耐药性监测哨点（SS）。采集了粪便样本（安大略省：ON、ON）和环境海绵拭子（不列颠哥伦比亚省：BC）。ON 和 ON 中的 流行率分别为 29%和 8%，BC 中为 7%。 。肯塔基州和 。利文斯顿是最常分离到的血清型，未检测到 。未在 BC 海绵拭子中检测到，但从 89%和 53%的安大略省粪便样本（ON 和 ON）中分离到。安大略省的 7 株对环丙沙星耐药。两种样本类型（98%）中的 流行率均较高。总的来说， 对四环素的耐药率在 26%到 69%之间。对头孢噻呋（=2 株）和庆大霉素（=2 株）的耐药率相对较低。在安大略省（10 种模式）和不列颠哥伦比亚省（14 种模式）的 中观察到了不同的耐药模式（不包括敏感分离株）。本研究表明，粪便样本更能为农场层面的病原体和 AMR 流行情况监测提供信息。未经进一步验证，海绵拭子在 检测方面的实用性有限，因此对公共卫生监测的作用有限。
