Del Rio Beatriz, Sánchez-Llana Esther, Martínez Noelia, Fernández María, Ladero Victor, Alvarez Miguel A
Department of Technology and Biotechnology of Dairy Products, Dairy Research Institute, IPLA-CSIC, Villaviciosa, Spain.
Molecular Microbiology Group, Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), Oviedo, Spain.
Front Microbiol. 2021 Jan 8;11:592172. doi: 10.3389/fmicb.2020.592172. eCollection 2020.
Enterococci are a diverse group of Gram-positive, lactic acid bacteria (LAB). They are found in many environments, including fermented foods, in which they could constitute a health threat since they produce biogenic amines, which consumption can lead to intoxication. Moreover, enterococci has also emerged as an important hospital-acquired pathogens via its acquisition of antimicrobial resistance. Bacteriophages possess features that make them optimal biotechnological weapons for controlling bacterial growth in disease and food spoilage contexts. However, no silver bullet bacteriophage exists that can eliminate all the undesirable bacteria in a complex environment. Rather, a combination of phages with different host ranges would be required which implies the need for large collections of diverse phages. This work reports the isolation of several -infecting bacteriophages from different types of cheese, along with the range of strains of diverse origin (from dairy to clinical environments) they are able to infect. The isolated phages showed a large diversity regarding the number and origin of strains they infect. Some of these phages were subjected to morphological and genomic characterization which confirmed their diversity and showed they belong to different families and genera. The present findings increase the potential arsenal for the bacteriophage-based biocontrol of harmful populations.
肠球菌是革兰氏阳性乳酸菌（LAB）的一个多样化群体。它们存在于许多环境中，包括发酵食品中，在这些环境中它们可能构成健康威胁，因为它们会产生生物胺，食用后可能导致中毒。此外，肠球菌还通过获得抗药性而成为重要的医院获得性病原体。噬菌体具有一些特性，使其成为在疾病和食品腐败环境中控制细菌生长的理想生物技术武器。然而，不存在能消除复杂环境中所有不良细菌的万能噬菌体。相反，需要不同宿主范围的噬菌体组合，这意味着需要大量多样的噬菌体库。这项工作报告了从不同类型奶酪中分离出几种感染肠球菌的噬菌体，以及它们能够感染的不同来源（从乳制品到临床环境）的肠球菌菌株范围。分离出的噬菌体在它们感染的菌株数量和来源方面表现出很大的多样性。其中一些噬菌体经过了形态学和基因组特征分析，证实了它们的多样性，并表明它们属于不同的科和属。目前的研究结果增加了基于噬菌体对有害肠球菌群体进行生物控制的潜在手段。
