Tessema Zenaw, Molla Yalew
Department of Pharmacy, College of Health Sciences, Debre Markos University, P. O. Box, 269, Debre Markos, Ethiopia.
Heliyon. 2021 Jan 19;7(1):e05901. doi: 10.1016/j.heliyon.2021.e05901. eCollection 2021 Jan.
Wounds are major problems of developing countries that can be managed alternatively using traditional medicinal plants. Since majority of currently available drugs for wound management are expensive and pose problems such as allergy and drug resistance, it is pivotal for the world to have intensified inquiries on the claimed medicinal plants to come up with wound healing chemicals being affordable, effective and safe. Ethiopian traditional healers recruit a wide range of medicinal plants with wound healing activities. Root bark juice of , the leaves of and are claimed among others in the folklore medicine. Therefore, the aim of this study was to evaluate the in vivo wound healing activities of the root bark juice of , the leaves of and in mice.
The root bark juice of , the leaves of and were collected, dried, ground to coarse powders. Then the crude extract was obtained by macerating with 80% methanol. The filtrate was dried, reconstituted in appropriate solvent and the wound healing activity was evaluated using excision and incision wound models.
On the last day of treatment, 80% methanol extracts from the selected medicinal plants showed a significant wound healing activity against control as supported by an increase in % wound contraction and a decrease in Epithelialization period. Ten percent of showed significant wound contraction against the control (P < 0.05) on days 2 and 4. But on day 6; except 5% extracts of and ; all doses of extracts contracted the wound significantly (P < 0.05). Extracts of Dodonea angustifolia (5% & 10%) and 10% of and increases wound contraction rate with increasing significant level on days 8 & 10 (P < 0.01) and 12 &14 (P < 0.001). Among the extracts, 10% of Dodonea angustifolia showed maximum percent (99.9%; P < 0.001) of wound contraction followed by 5% Dodonea angustifolia (99.15%; P < 0.001) and 10% (99.00%; P < 0.001) on the last day of treatment. In addition; significantly shorter healing time was attained by 5% &10% leaves of Dodonea angustifolia (P < 0.01), 10% leaves & root barks (P < 0.05). Ten percent of & (P < 0.05) and both doses (5% & 10%) of Dodonea angustifolia (P < 0.01) significantly increased the tensile strength by 54.10%, 56.58%, 63.04%, and 79.19%, respectively against the control.
The 80% methanol crude extracts of the study plants support the traditional claims for healing of wounds as evidenced by an increase in wound contraction rate and tensile strength, decrease in Epithelialization period.
伤口是发展中国家面临的主要问题，可使用传统药用植物进行替代性处理。由于目前大多数用于伤口处理的药物价格昂贵，且存在过敏和耐药性等问题，因此全球迫切需要加强对声称具有药用价值的植物的研究，以开发出价格亲民、有效且安全的伤口愈合化学品。埃塞俄比亚的传统治疗师使用多种具有伤口愈合活性的药用植物。在民间医学中，[植物名称1]的根皮汁、[植物名称2]和[植物名称3]的叶子等都有相关记载。因此，本研究旨在评估[植物名称1]的根皮汁、[植物名称2]和[植物名称3]的叶子在小鼠体内的伤口愈合活性。
收集[植物名称1]的根皮汁、[植物名称2]和[植物名称3]的叶子，干燥后研磨成粗粉。然后用80%甲醇浸渍得到粗提取物。将滤液干燥，用适当溶剂复溶，并用切除和切开伤口模型评估伤口愈合活性。
在治疗的最后一天，所选药用植物的80%甲醇提取物显示出显著的伤口愈合活性，与对照组相比，伤口收缩百分比增加，上皮化期缩短。10%的[植物名称1]在第2天和第4天与对照组相比显示出显著的伤口收缩（P < 0.05）。但在第6天，除了5%的[植物名称2]和[植物名称3]提取物外，所有剂量的提取物均使伤口显著收缩（P < 0.05）。狭叶坡柳（5%和10%）以及10%的[植物名称2]和[植物名称3]的提取物在第8天和第10天（P < 0.01）以及第12天和第14天（P < 0.001）随着显著水平的增加而提高伤口收缩率。在提取物中，10%的狭叶坡柳在治疗的最后一天显示出最大的伤口收缩百分比（99.9%；P < 0.001），其次是5%的狭叶坡柳（99.15%；P < 0.001）和10%的[植物名称2]（99.00%；P < 0.001）。此外，5%和10%的狭叶坡柳叶子（P < 0.01）、10%的[植物名称2]叶子和[植物名称1]根皮（P < 0.05）显著缩短了愈合时间。10%的[植物名称2]和[植物名称3]（P < 0.05）以及狭叶坡柳的两种剂量（5%和10%）（P < 0.01）与对照组相比，分别使抗张强度显著提高了54.10%、56.58%、63.04%和79.19%。
研究植物的80%甲醇粗提取物支持了其用于伤口愈合的传统说法，表现为伤口收缩率和抗张强度增加，上皮化期缩短。
