Thomason J L, Gelbart S M, Wilcoski L M, Peterson A K, Jilly B J, Hamilton P R
Department of Obstetrics and Gynecology, University of Wisconsin, Milwaukee Campus.
Obstet Gynecol. 1988 Apr;71(4):607-11.
Two biochemical indicators of bacterial vaginosis, proline aminopeptidase activity and gas-liquid chromatographic analysis, were compared. Five hundred women had their vaginal secretions tested for pH, presence of a positive amine test, levels of volatile and nonvolatile short-chain organic acids, and proline aminopeptidase activity. In addition, direct microscopic and Gram stain examinations were performed. Of the 500 women, 349 (70%) had some form of vaginitis. One hundred sixteen were diagnosed as having bacterial vaginosis, and 69 of these (59%) had Mobiluncus sp on either direct microscopic or Gram stain examination. Two hundred thirty-three had either mixed or other forms of vaginitis. One hundred fifty-one patients were normal. The sensitivity of the proline aminopeptidase assay was 83 and 79%, respectively, in patients having bacterial vaginosis with and without Mobiluncus morphotypes. In contrast, gas-liquid chromatography of short-chain organic acids had sensitivities of 71 and 30%, respectively. Specificity of both assays was about 95%. The greater sensitivity of the proline aminopeptidase assay, especially in patients without Mobiluncus morphotypes, proves its superiority.
对细菌性阴道病的两种生化指标——脯氨酸氨基肽酶活性和气-液色谱分析进行了比较。500名女性接受了阴道分泌物的pH值、胺试验阳性情况、挥发性和非挥发性短链有机酸水平以及脯氨酸氨基肽酶活性检测。此外，还进行了直接显微镜检查和革兰氏染色检查。在这500名女性中，349名（70%）患有某种形式的阴道炎。116名被诊断为患有细菌性阴道病，其中69名（59%）在直接显微镜检查或革兰氏染色检查中发现有动弯杆菌属。233名患有混合性或其他形式的阴道炎。151名患者正常。脯氨酸氨基肽酶检测在患有和未患有动弯杆菌形态型的细菌性阴道病患者中的敏感性分别为83%和79%。相比之下，短链有机酸的气-液色谱分析敏感性分别为71%和30%。两种检测方法的特异性均约为95%。脯氨酸氨基肽酶检测具有更高的敏感性，尤其是在没有动弯杆菌形态型的患者中，证明了其优越性。
