Belay N, Mukhopadhyay B, Conway de Macario E, Galask R, Daniels L
Department of Microbiology, University of Iowa, Iowa City 52242.
J Clin Microbiol. 1990 Jul;28(7):1666-8. doi: 10.1128/jcm.28.7.1666-1668.1990.
Twelve vaginal samples were collected from separate patients, processed anaerobically, and added to methanogenic enrichment medium. Methanogenic activity was detected in two samples, both of which were from patients with bacterial vaginosis. None of the samples from healthy patients yielded positive methanogen cultures. One sample from a patient with bacterial vaginosis did not show any detectable methanogenic activity. Two methanogen isolates were obtained from one of the methanogen-positive samples, and both were identified as Methanobrevibacter smithii on the basis of morphological, cultural, and immunological features.
从不同患者身上采集了12份阴道样本，进行厌氧处理后，添加到产甲烷富集培养基中。在两份样本中检测到了产甲烷活性，这两份样本均来自患有细菌性阴道病的患者。健康患者的样本均未培养出阳性产甲烷菌。一份来自细菌性阴道病患者的样本未显示出任何可检测到的产甲烷活性。从一份产甲烷菌阳性样本中获得了两株产甲烷菌分离株，根据形态、培养和免疫学特征，这两株菌均被鉴定为史氏甲烷短杆菌。
