Department of Plastic Surgery, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, Zhejiang Province, P.R. China.
Department of Plastic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, P.R. China.
J Appl Biomater Funct Mater. 2021 Jan-Dec;19:2280800021989698. doi: 10.1177/2280800021989698.
The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by and . Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.
本研究旨在制备一种新型壳聚糖-胶原蛋白海绵（CCS），用于潜在的伤口敷料应用。CCS 通过冷冻干燥由 3.0%的壳聚糖混合物与 1.0%的 I 型胶原蛋白（7:3（w/w））制备而成。然后，通过一系列测试来评估敷料的性能。新型敷料对 NIH3T3 细胞表现出安全性。此外，CCS 显示出比空对照物（接种了 和 ）更大的显著周围抑制区。此外，CCS 的水分率比胶原蛋白和空白海绵组增加得更快。结果表明，CCS 具有无毒、生物相容性、良好的抗菌活性和保水特性。我们使用全厚度切除伤口愈合模型来评估新型敷料的体内功效。结果表明，与仅用胶原蛋白作为阴性对照的损伤相比，在术后第 14 天，用壳聚糖-胶原蛋白处理的伤口具有显著的愈合效果。我们的结果表明，壳聚糖-胶原蛋白伤口敷料被鉴定为进一步伤口应用的一种有前途的新型候选物。
