Dalmon Anne, Diévart Virgine, Thomasson Maxime, Fouque Romain, Vaissière Bernard E, Guilbaud Laurent, Le Conte Yves, Henry Mickaël
INRAE-Unité Abeilles et Environnement-Site Agroparc-Domaine St Paul-228, Route de l'aérodrome CS40509, CEDEX 9, 84914 Avignon, France.
Insects. 2021 Jan 29;12(2):122. doi: 10.3390/insects12020122.
Viruses are known to contribute to bee population decline. Possible spillover is suspected from the co-occurrence of viruses in wild bees and honey bees. In order to study the risk of virus transmission between wild and managed bee species sharing the same floral resource, we tried to maximize the possible cross-infections using which is highly attractive to honey bees and a broad range of wild bee species. Virus prevalence was compared over two years in Southern France. A total of 1137 wild bees from 29 wild bee species (based on COI barcoding) and 920 honey bees () were checked for the seven most common honey bee RNA viruses. Halictid bees were the most abundant. Co-infections were frequent, and (SBV), (BQCV), (ABPV) and (IAPV) were widespread in the hymenopteran pollinator community. Conversely, (DWV) was detected at low levels in wild bees, whereas it was highly prevalent in honey bees (78.3% of the samples). Both wild bee and honey bee virus isolates were sequenced to look for possible host-specificity or geographical structuring. ABPV phylogeny suggested a specific cluster for bees, while isolates of DWV from bumble bees ( spp.) clustered together with honey bee isolates, suggesting a possible spillover.
已知病毒会导致蜜蜂数量减少。野生蜜蜂和蜜蜂中病毒的共同存在引发了对可能的病毒溢出的怀疑。为了研究共享相同花卉资源的野生蜜蜂和养殖蜜蜂物种之间病毒传播的风险，我们试图通过使用对蜜蜂和多种野生蜜蜂物种极具吸引力的[未提及内容]来最大化可能的交叉感染。在法国南部对病毒流行情况进行了两年的比较。对来自29种野生蜜蜂物种（基于细胞色素氧化酶亚基I条形码）的1137只野生蜜蜂和920只蜜蜂（[未提及内容]）进行了七种最常见的蜜蜂RNA病毒检测。隧蜂是数量最多的。共感染很常见，黑蜂王台病毒（SBV）、黑蜂病毒（BQCV）、急性蜜蜂麻痹病毒（ABPV）和以色列急性麻痹病毒（IAPV）在膜翅目传粉者群落中广泛存在。相反，残翅病毒（DWV）在野生蜜蜂中检测到的水平较低，而在蜜蜂中高度流行（78.3%的样本）。对野生蜜蜂和蜜蜂的病毒分离株都进行了测序，以寻找可能的宿主特异性或地理结构。ABPV系统发育表明[未提及蜜蜂种类]存在特定聚类群，而来自熊蜂（[未提及种类]）的DWV分离株与蜜蜂分离株聚集在一起，表明可能存在溢出。
