Wang Zhenzhen, Song Deguang, Wang Gaili, Li Caili, Liu Xueli, Wang Xinran, Li Zi, Guan Jiyu, Zhao Kui, He Wenqi, Gao Feng, Lan Yungang
Key Laboratory ofZoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China.
Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun, Jilin, China.
Vet Microbiol. 2021 Apr;255:109015. doi: 10.1016/j.vetmic.2021.109015. Epub 2021 Feb 17.
Porcine hemagglutinating encephalomyelitis virus (PHEV) displays neurotropism and induces atypical autophagy. However, the exact mechanisms mediating autophagy induced by PHEV remains uncharacterized. Transcription factor EB (TFEB) is a master transcriptional regulator playing a key role in autophagy and its activity is regulated by MTORC1 kinase on the surface of lysosomes. We first found that PHEV infection decreases TFEB expression, while it activates TFEB by inhibiting MTORC1 activation, indicating that TFEB plays a complex role in the process of PHEV-induced autophagy through opposite regulation of its expression and activity. Furthermore, this study preliminarily demonstrated that PHEV replication is dependent on TFEB expression.
猪血凝性脑脊髓炎病毒（PHEV）具有嗜神经性，并可诱导非典型自噬。然而，介导PHEV诱导自噬的确切机制仍不清楚。转录因子EB（TFEB）是一种主要的转录调节因子，在自噬中起关键作用，其活性受溶酶体表面的MTORC1激酶调节。我们首次发现，PHEV感染会降低TFEB的表达，同时通过抑制MTORC1的激活来激活TFEB，这表明TFEB在PHEV诱导的自噬过程中通过对其表达和活性的相反调节发挥复杂作用。此外，本研究初步证明PHEV复制依赖于TFEB的表达。
