Department of Nanoscience, Graduate School of Natural and Applied Science, Atatürk University, Erzurum, Turkey.
Aquatic Biotechnology Laboratory, Faculty of Fisheries, Atatürk University, Erzurum, Turkey.
Eat Weight Disord. 2022 Feb;27(1):163-177. doi: 10.1007/s40519-021-01140-5. Epub 2021 Mar 12.
Anxiety and obesity are two current phenomena. They are among the important public health problems with increasing prevalence worldwide. Although it is claimed that there are strong relations between them, the mechanism of this relationship has not been fully clarified yet. On the other hand, the effect of this relationship on the offspring has been another research subject. In this study, obese zebrafish were obtained by feeding two different diets, one containing high amount of lipid (HF) and the other containing high amount of carbohydrate (HK), and their anxiety levels were evaluated. To establish a relationship between these two phenomena, in addition to histopathological and immunohistochemical analysis in the brain tissues of fish, the transcription levels of some genes related to lipid and carbohydrate metabolisms were determined. In addition, offspring were taken from obese zebrafish and studied to examine the effect of parental obesity on offspring. As a result, it was observed that the HC diet, causing more weight increase than the HF diet, showed an anxiolytic while the HF diet an anxiogenic effect. It was suggested that the probable cause of this situation may be the regulatory effect on the appetite-related genes depending on the upregulation severity of the PPAR gene family based on the diet content. In addition, it was also suggested that it may have contributed to this process in neuron degenerations caused by oxidative stress. Regarding effects on offspring, it can be concluded that HF diet-induced obesity has more negative effects on the next generation than the HC diet.Level of evidenceNo Level of evidence: animal study.
焦虑和肥胖是当前的两种现象。它们是全球范围内日益流行的重要公共卫生问题之一。尽管有人声称它们之间存在很强的关系，但这种关系的机制尚未完全阐明。另一方面，这种关系对后代的影响也是另一个研究课题。在这项研究中，通过给两种不同的饮食喂养斑马鱼来获得肥胖的斑马鱼，一种饮食含有高量的脂肪（HF），另一种饮食含有高量的碳水化合物（HK），并评估它们的焦虑水平。为了建立这两种现象之间的关系，除了对鱼脑组织进行组织病理学和免疫组织化学分析外，还确定了一些与脂肪和碳水化合物代谢相关的基因的转录水平。此外，还从肥胖的斑马鱼中取出后代进行研究，以检查父母肥胖对后代的影响。结果表明，与 HF 饮食相比，导致体重增加更多的 HC 饮食表现出抗焦虑作用，而 HF 饮食则表现出焦虑作用。据推测，这种情况的可能原因可能是基于饮食内容，根据 PPAR 基因家族的上调严重程度，对与食欲相关的基因产生调节作用。此外，还表明它可能在氧化应激引起的神经元退化中对此过程有贡献。关于对后代的影响，可以得出结论，HF 饮食诱导的肥胖对下一代的负面影响大于 HC 饮食。证据水平无证据等级：动物研究。
