Chair of Chemistry of Biogenic Resources, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Schulgasse 16, 94315, Straubing, Germany.
Synbiofoundry@TUM, Technical University of Munich, Schulgasse 22, 94315, Straubing, Germany.
Angew Chem Int Ed Engl. 2021 Jun 21;60(26):14701-14706. doi: 10.1002/anie.202017027. Epub 2021 May 10.
Here we report a new robust nicotinamide dinucleotide phosphate cofactor analog (carba-NADP ) and its acceptance by many enzymes in the class of oxidoreductases. Replacing one ribose oxygen with a methylene group of the natural NADP was found to enhance stability dramatically. Decomposition experiments at moderate and high temperatures with the cofactors showed a drastic increase in half-life time at elevated temperatures since it significantly disfavors hydrolysis of the pyridinium-N-glycoside bond. Overall, more than 27 different oxidoreductases were successfully tested, and a thorough analytical characterization and comparison is given. The cofactor carba-NADP opens up the field of redox-biocatalysis under harsh conditions.
在这里，我们报告了一种新的稳定的烟酰胺腺嘌呤二核苷酸磷酸辅酶类似物（碳氮二磷酸 NADP），以及它被许多氧化还原酶类酶接受。我们发现，用亚甲基取代天然 NADP 中的一个核糖氧原子可以显著提高其稳定性。在中等和高温下进行的分解实验表明，由于其显著抑制了吡啶-N-糖苷键的水解，因此在高温下半衰期显著延长。总的来说，我们成功地测试了 27 种以上的氧化还原酶，并对其进行了全面的分析表征和比较。该辅酶碳氮二磷酸 NADP 为苛刻条件下的氧化还原生物催化开辟了新的领域。
