Liang Shuzhang, Cao Yuqing, Dai Yuguo, Wang Fenghui, Bai Xue, Song Bin, Zhang Chaonan, Gan Chunyuan, Arai Fumihito, Feng Lin
School of Mechanical Engineering & Automation, Beihang University, Beijing 100191, China.
BEIGE Institue of Robot & Intelligent Manufacturing, Weifang 261000, China.
Micromachines (Basel). 2021 Mar 6;12(3):271. doi: 10.3390/mi12030271.
Non-contact manipulation technology has a wide range of applications in the manipulation and fabrication of micro/nanomaterials. However, the manipulation devices are often complex, operated only by professionals, and limited by a single manipulation function. Here, we propose a simple versatile optoelectronic tweezer (OET) system that can be easily controlled for manipulating microparticles with different sizes. In this work, we designed and established an optoelectronic tweezer manipulation system. The OET system could be used to manipulate particles with a wide range of sizes from 2 μm to 150 μm. The system could also manipulate micro-objects of different dimensions like 1D spherical polystyrene microspheres, 2D rod-shaped euglena gracilis, and 3D spiral microspirulina. Optical microscopic patterns for trapping, storing, parallel transporting, and patterning microparticles were designed for versatile manipulation. The sorting, rotation, and assembly of single particles in a given region were experimentally demonstrated. In addition, temperatures measured under different objective lenses indicate that the system does not generate excessive heat to damage bioparticles. The non-contact versatile manipulation reduces operating process and contamination. In future work, the simple optoelectronic tweezers system can be used to control non-contaminated cell interaction and micro-nano manipulation.
非接触式操控技术在微纳材料的操控与制造中有着广泛应用。然而，操控设备往往较为复杂，仅由专业人员操作，且受单一操控功能限制。在此，我们提出一种简单通用的光电镊子（OET）系统，它能够轻松控制，用于操控不同尺寸的微粒。在这项工作中，我们设计并建立了一个光电镊子操控系统。该OET系统可用于操控尺寸范围从2μm到150μm的各种微粒。该系统还能操控不同维度的微物体，如1D球形聚苯乙烯微球、2D杆状纤细裸藻以及3D螺旋形微螺旋藻。为实现通用操控，设计了用于捕获、存储、并行传输和图案化微粒的光学显微镜图案。通过实验展示了在给定区域内单个粒子的分选、旋转和组装。此外，在不同物镜下测量的温度表明该系统不会产生过多热量来损坏生物粒子。非接触式通用操控减少了操作过程和污染。在未来工作中，这种简单的光电镊子系统可用于控制无污染的细胞相互作用和微纳操控。
