Abdel-Latif Hany M R, Shukry Mustafa, El Euony Omnia I, Mohamed Soliman Mohamed, Noreldin Ahmed E, Ghetas Hanan A, Dawood Mahmoud A O, Khallaf Mohamed A
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, Egypt.
Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt.
Biology (Basel). 2021 Mar 2;10(3):183. doi: 10.3390/biology10030183.
The current investigation assessed the impacts of sub-lethal concentrations of silicon dioxide nanoparticles (SiONPs) on hepato-renal functions, histopathological characteristics, and gene transcription in gills and liver of Nile tilapia juveniles. Fish were exposed to 20, 40, and 100 mg/L of SiONPs for 3 weeks. Pairwise comparisons with the control group showed a significant dose-dependent elevation in serum ALP, ALT, and AST enzyme activities as well as blood urea and creatinine levels in SiONP-intoxicated groups. Exposure to 100 mg/L SiONPs significantly upregulated expression of , , , and genes in the gills as compared to the control group. Moreover, exposure to 100 mg/L SiONPs significantly upregulated the expression , , , , and genes in the hepatic tissues as compared to the control group. Exposure of fish to 20 mg SiONPs/L significantly increased the mRNA expression levels of in both the gills and liver tissues. Notably, all tested SiONP concentrations significantly upregulated the transcription of gene in gills and liver of Nile tilapia as compared to the control group. Interestingly, varying histopathological alterations in renal, hepatopancreatic, and branchial tissues were observed to be correlated to the tested SiONP concentrations. In conclusion, our results provide additional information on the toxic impacts of SiONPs in Nile tilapia at the hematological, tissue, and molecular levels.
当前的研究评估了亚致死浓度的二氧化硅纳米颗粒（SiONPs）对尼罗罗非鱼幼鱼肝肾 功能、组织病理学特征以及鳃和肝脏中基因转录的影响。将鱼暴露于20、40和100 mg/L的SiONPs中3周。与对照组的两两比较显示，SiONP中毒组的血清碱性磷酸酶（ALP）、谷丙转氨酶（ALT）和谷草转氨酶（AST）酶活性以及血尿素和肌酐水平呈显著的剂量依赖性升高。与对照组相比，暴露于100 mg/L SiONPs显著上调了鳃中 、 、 和 基因的表达。此外，与对照组相比，暴露于100 mg/L SiONPs显著上调了肝组织中 、 、 、 和 基因的表达。将鱼暴露于20 mg SiONPs/L显著增加了鳃和肝组织中 的mRNA表达水平。值得注意的是，与对照组相比，所有测试的SiONP浓度均显著上调了尼罗罗非鱼鳃和肝脏中 基因的转录。有趣的是，观察到肾、肝胰腺和鳃组织中不同的组织病理学改变与测试的SiONP浓度相关。总之，我们的结果提供了关于SiONPs对尼罗罗非鱼在血液学、组织和分子水平上的毒性影响的更多信息。
