Michalska-Sionkowska Marta, Warżyńska Oliwia, Kaczmarek-Szczepańska Beata, Łukowicz Krzysztof, Osyczka Anna Maria, Walczak Maciej
Department of Environmental Microbiology and Biotechnology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.
Department of Biomaterials and Cosmetics Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland.
Materials (Basel). 2021 Mar 10;14(6):1322. doi: 10.3390/ma14061322.
Collagen possesses unique properties, e.g., biocompatibility, biodegradability, and non-toxicity. However, collagen material degrades too quickly and has low mechanical properties. One of the methods of polymers' modification is mixing them to obtain blends. In this study, the influence of β-glucan for collagen material was analyzed. The interaction between the functional groups of the polymer was analyzed by ATR-FTIR (attenuated total reflection-fourier transform infrared) spectroscopy. The influence of β-glucan on mechanical properties was evaluated. The surface properties of materials were assessed using contact angle measurements and the topography of materials was evaluated by AFM (atomic force microscope). The structure of materials was analyzed according to SEM (scanning electron microscopy) pictures. Moreover, the DPPH-free radicals' scavenging ability and biocompatibility against erythrocytes and HaCaT cells were evaluated. Collagen and β-glucan were bound together by a hydrogen bond. β-glucan addition increased the roughness of the surface of the film and resulted in a more rigid character of the materials. A small addition of β-glucan to collagen provided a more hydrophilic character. All the materials could swell in in vitro conditions and showed antioxidant activity. Materials do not cause erythrocyte hemolysis. Finely, our cytotoxicity studies indicated that β-glucan can be safely added at small (10% or less) quantity to collagen matrix, they sufficiently support cell growth, and the degradation products of such matrices may actually provide some beneficial effects to the surrounding cells/tissues.
胶原蛋白具有独特的性质，例如生物相容性、生物降解性和无毒性。然而，胶原蛋白材料降解太快且机械性能较低。聚合物改性的方法之一是将它们混合以获得共混物。在本研究中，分析了β-葡聚糖对胶原蛋白材料的影响。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）分析聚合物官能团之间的相互作用。评估了β-葡聚糖对机械性能的影响。使用接触角测量评估材料的表面性质，并通过原子力显微镜（AFM）评估材料的形貌。根据扫描电子显微镜（SEM）图片分析材料的结构。此外，评估了材料对DPPH自由基的清除能力以及对红细胞和HaCaT细胞的生物相容性。胶原蛋白和β-葡聚糖通过氢键结合在一起。添加β-葡聚糖增加了薄膜表面的粗糙度，并使材料具有更刚性的特性。向胶原蛋白中少量添加β-葡聚糖可使其具有更亲水的特性。所有材料在体外条件下都能膨胀并表现出抗氧化活性。材料不会引起红细胞溶血。最后，我们的细胞毒性研究表明，β-葡聚糖可以以少量（10%或更少）安全地添加到胶原蛋白基质中，它们能充分支持细胞生长，并且这种基质的降解产物实际上可能对周围细胞/组织产生一些有益影响。
