Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 60 Let Oktjabrja pr-t, 7, Bld. 2, 117312, Moscow, Russian Federation.
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the RAS, Federal Research Centre "Pushchino Scientific Centre of Biological Investigations RAS", 142290, Pushchino, Russian Federation.
Arch Microbiol. 2023 May 11;205(6):232. doi: 10.1007/s00203-023-03577-x.
A Gram-negative, strictly aerobic, chemoorganotrophic, bacteriochlorophyll a-containing, slow-growing bacterium was isolated from the lichen Flavocetraria nivalis and designated strain BP6-180914. Cells of this strain were large nonmotile rods, which reproduced by binary fission. Cells grew under oxic conditions and were able to utilize sugars and several polysaccharides, including starch and pectin. Strain BP6-180914 was psychrotolerant and moderately acidophilic growing at 4-35 °C (optimum 20-28 °C) and between pH 4.0 and 7.5 (optimum 4.5-5.5). The major fatty acids were Cω7c, C cyclo, C and C The polar lipids were diphosphatidylglycerols, phosphatidylglycerols, phosphatidylethanolamines, phosphatidylcholines, unidentified aminolipids, and a number of glycolipids, the major one being an unidentified glycolipid. The quinone was Q-10. The DNA G + C content was 63.65%. Comparative 16S rRNA gene sequence analysis revealed that strain BP6-180914 was a member of the order Hyphomicrobiales and belonged to the family Lichenihabitantaceae defined by the lichen-dwelling facultative aerobic chemo-organotroph Lichenihabitans psoromatis (92.7% sequence similarity). The results of phylogenomic and genomic relatedness analyses showed that strain BP6-180914 could clearly be distinguished from other species in the order Hyphomicrobiales with average nucleotide identity values of < 74.05% and genome-to-genome distance values of < 21.1%. The AAI value of 65.9% between strain BP6-180914 and L. psoromatis allowed us to assign this strain to the novel genus of the family Lichenihabitantaceae. Therefore, it is proposed that strain BP6-180914 represents a novel species in a new genus, Lichenifustis flavocetrariae gen. nov., sp. nov.; strain BP6-180914 (= KCTC 92872 = VKM B-3641 = UQM 41506) is the type strain.
一株革兰氏阴性、严格需氧、化能有机营养、含细菌叶绿素 a、生长缓慢的细菌，从地衣 Flavocetraria nivalis 中分离得到，并被命名为 BP6-180914 株。该菌株的细胞为大型非运动杆状，通过二分分裂繁殖。细胞在需氧条件下生长，能够利用糖和几种多糖，包括淀粉和果胶。BP6-180914 株为嗜冷菌，中度嗜酸，生长温度为 4-35°C（最适温度为 20-28°C），pH 值为 4.0-7.5（最适 pH 值为 4.5-5.5）。主要脂肪酸为 Cω7c、C 环、C 和 C 极性脂类为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、未鉴定的胺脂类和许多糖脂类，主要的糖脂类是一种未鉴定的糖脂类。醌类为 Q-10。DNA G+C 含量为 63.65%。比较 16S rRNA 基因序列分析表明，BP6-180914 株是 Hyphomicrobiales 目成员，属于 Lichenihabitantaceae 科，由地衣居住的兼性需氧化能有机营养型地衣 Habitans psoromatis 定义（92.7%的序列相似性）。系统发育基因组和基因组相关性分析的结果表明，BP6-180914 株与 Hyphomicrobiales 目中的其他种明显不同，平均核苷酸同一性值<74.05%，基因组到基因组的距离值<21.1%。BP6-180914 株与 L. psoromatis 的 AAI 值为 65.9%，使我们能够将该菌株归入 Lichenihabitantaceae 科的新属。因此，建议将 BP6-180914 株命名为新属地衣 Fustis flavocetrariae gen. nov. 的新种，即地衣 Flavocetrariae nov. 种 nov.；BP6-180914 株（=KCTC 92872=VKM B-3641=UQM 41506）是模式株。
