Instituto de Biología Molecular y Celular de Plantas, Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain.
Molecules. 2021 Mar 24;26(7):1818. doi: 10.3390/molecules26071818.
New strategies of control need to be developed with the aim of economic and environmental sustainability in plant and crop protection. Metabolomics is an excellent platform for both understanding the complex plant-pathogen interactions and unraveling new chemical control strategies. GC-MS-based metabolomics, along with a phytohormone analysis of a compatible and incompatible interaction between tomato plants and f. sp. revealed the specific volatile chemical composition and the plant signals associated with them. The susceptible tomato plants were characterized by the over-emission of methyl- and ethyl-salicylate as well as some fatty acid derivatives, along with an activation of salicylic acid and abscisic acid signaling. In contrast, terpenoids, benzenoids, and 2-ethylhexanoic acid were differentially emitted by plants undergoing an incompatible interaction, together with the activation of the jasmonic acid (JA) pathway. In accordance with this response, a higher expression of several genes participating in the biosynthesis of these volatiles, such as , and , as well as , a JA marker gene, was found to be induced by the fungus in these resistant plants. The characterized metabolome of the immune tomato plants could lead to the development of new resistance inducers against wilt treatment.
需要开发新的控制策略，以实现植物和作物保护的经济和环境可持续性。代谢组学是理解复杂的植物-病原体相互作用和揭示新的化学控制策略的绝佳平台。基于 GC-MS 的代谢组学，以及番茄植株与 f. sp. 之间相容和不相容相互作用的植物激素分析，揭示了与特定挥发性化学组成和植物信号相关的特定挥发性化学组成和植物信号。易感番茄植株的特征是甲基和乙基水杨酸以及一些脂肪酸衍生物的过度排放，以及水杨酸和脱落酸信号的激活。相比之下，在经历不相容相互作用的植物中，萜类、苯类和 2-乙基己酸以及茉莉酸（JA）途径的激活会有差异地排放。与此响应一致，发现参与这些挥发性物质生物合成的几个基因的表达更高，如 、 、 以及 JA 标记基因，在这些抗性植物中被真菌诱导。免疫番茄植株的特征代谢组学可能会导致开发针对 枯萎病 的新抗性诱导剂。
