Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA.
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, USA.
Curr Opin Microbiol. 2021 Jun;61:67-72. doi: 10.1016/j.mib.2021.03.004. Epub 2021 Apr 1.
Bacterial microcompartments (BMCs) with selectively permeable shells and encapsulated enzyme cores are well-suited candidates for nano-bioreactors because of their advantages of enhancing pathway flux and protection against toxic products. To better study and engineer protein-based BMCs, a series of protein chemistry approaches are adopted. As one of the most advanced techniques, genetic code expansion can introduce various noncanonical amino acids (ncAAs) with diverse functional groups into target proteins, thus providing powerful tools for protein studies and engineering. This review summarizes and proposes useful tools based on current development of the genetic code expansion technique towards challenges in BMC studies and engineering.
细菌微隔间（BMCs）具有选择性渗透的外壳和包裹的酶核心，是纳米生物反应器的理想候选物，因为它们具有增强途径通量和防止有毒产物的优势。为了更好地研究和设计基于蛋白质的 BMCs，采用了一系列蛋白质化学方法。作为最先进的技术之一，遗传密码扩展可以将各种具有不同功能基团的非标准氨基酸（ncAAs）引入目标蛋白质中，从而为蛋白质研究和工程提供了强大的工具。本综述总结并提出了基于遗传密码扩展技术当前发展的有用工具，以应对 BMC 研究和工程中的挑战。
