Kulesza Kamila, Biedunkiewicz Anna, Nowacka Karolina, Dynowska Maria, Urbaniak Monika, Stępień Łukasz
Department of Microbiology and Mycology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland.
Plant-Pathogen Interaction Team, Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics of the Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Pathogens. 2021 Apr 8;10(4):446. doi: 10.3390/pathogens10040446.
The study aimed to compare the yeast species diversity in the specific environment of dishwashers, taking into account the potential risk for users. Yeasts were isolated from ten dishwashers and from tap water supplied to the appliances. Samples were collected for mycological analyses at the beginning of each month, from February to May 2016. Four dishwasher sites (rubber seals, detergent dispensers, sprinklers, and water drains) were analyzed. The microfungi were identified by the standard procedures applied in mycological diagnostics. To confirm species identification, molecular analysis was performed based on the sequences of the D1/D2 region. The presence of microfungi was detected in 70% of the investigated appliances. Rubber seals, detergent dispensers, and water drains were the most frequently colonized elements. Thirty-five yeast strains were isolated in this study, of which twenty-seven were obtained from dishwashers and eight from tap water. The strains belonged to six genera and six species (, and ). Most of the strains came from rubber seals. In this way, it was demonstrated that the dishwashers' condition is sufficient as an ecological niche for microfungi.
该研究旨在比较洗碗机特定环境中的酵母种类多样性，并考虑对用户的潜在风险。从十台洗碗机以及供应给这些电器的自来水中分离出酵母。于2016年2月至5月每月初采集样本进行真菌学分析。对四个洗碗机部位（橡胶密封圈、洗涤剂分配器、喷头和排水口）进行了分析。通过真菌学诊断中应用的标准程序鉴定微真菌。为确认物种鉴定，基于D1/D2区域的序列进行了分子分析。在70%的被调查电器中检测到了微真菌。橡胶密封圈、洗涤剂分配器和排水口是最常被定植的部件。本研究分离出35株酵母菌株，其中27株来自洗碗机，8株来自自来水。这些菌株属于六个属和六个种（ 、 、 、 、 、 ）。大多数菌株来自橡胶密封圈。由此证明，洗碗机的环境足以作为微真菌的生态位。
