Department of Bioinformatics and Biological Statistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Center for Biotechnology and Microbiology, University of Swat, Swat, KP, Pakistan.
Comput Biol Med. 2021 Jun;133:104420. doi: 10.1016/j.compbiomed.2021.104420. Epub 2021 Apr 24.
Reports of the novel and more contagious strains of SARS-CoV-2 originating in different countries have further aggravated the pandemic situation. The recent substitutions in spike protein may be critical for the virus to evade the host's immune system and therapeutics that have already been developed. Thus, this study has employed an immunoinformatics pipeline to target the spike protein of this novel strain to construct an immunogenic epitope (CTL, HTL, and B cell) vaccine against the new variant. Our investigation revealed that 12 different epitopes imparted a critical role in immune response induction. This was validated by an exploration of physiochemical properties and experimental feasibility. In silico and host immune simulation confirmed the expression and induction of both primary and secondary immune factors such as IL, cytokines, and antibodies. The current study warrants further lab experiments to demonstrate its efficacy and safety.
有关源自不同国家的新型、更具传染性的 SARS-CoV-2 变异株的报告，使大流行形势进一步恶化。刺突蛋白的最近突变可能对病毒逃避宿主免疫系统和已开发的治疗方法至关重要。因此，本研究采用免疫信息学方法针对新型变异株的刺突蛋白，构建针对新型变异株的免疫原性表位（CTL、HTL 和 B 细胞）疫苗。我们的研究表明，12 种不同的表位在免疫反应诱导中起关键作用。通过对理化性质和实验可行性的探索，验证了这一点。在计算机和宿主免疫模拟中，确认了 IL、细胞因子和抗体等主要和次要免疫因子的表达和诱导。本研究需要进一步的实验室实验来证明其疗效和安全性。
