AlphaFold2 预测了多药转运蛋白 LmrP 的内向构象。
AlphaFold2 predicts the inward-facing conformation of the multidrug transporter LmrP.
机构信息
Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles, Brussels, Belgium.
出版信息
Proteins. 2021 Sep;89(9):1226-1228. doi: 10.1002/prot.26138. Epub 2021 May 18.
Abstract
As part of the CASP competition, the protein structure prediction algorithm AlphaFold2 generated multiple models of the proton/drug antiporter LmrP. Previous distance restraints from double electron-electron resonance spectroscopy, a technique which reports distance distributions between spin labels attached to proteins, suggest that one of the lower-ranked models may have captured a conformation that has so far eluded experimental structure determination.
摘要
作为 CASP 竞赛的一部分，蛋白质结构预测算法 AlphaFold2 生成了质子/药物反向转运蛋白 LmrP 的多个模型。来自双电子电子共振光谱学的先前距离约束的技术，报告了附着在蛋白质上的自旋标记之间的距离分布，表明排名较低的模型之一可能捕获了一种迄今为止逃避实验结构确定的构象。
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