Fraiture Marie-Alice, Coucke Wim, Pol Morgane, Rousset Dominique, Gourinat Ann-Claire, Biron Antoine, Broeders Sylvia, Vandermassen Els, Dupont-Rouzeyrol Myrielle, Roosens Nancy H C
Transversal & Applied Genomics (TAG), Sciensano, rue Juliette Wytsman 14, 1050 Brussels, Belgium.
Quality of Laboratories, Sciensano, rue Juliette Wytsman 14, 1050 Brussels, Belgium.
Microorganisms. 2021 Jun 16;9(6):1312. doi: 10.3390/microorganisms9061312.
Zika virus, an arbovirus responsible for major outbreaks, can cause serious health issues, such as neurological diseases. In the present study, different types of samples (serum, saliva, and urine), collected in 2015-2016 in New Caledonia and French Guiana from 53 patients presenting symptoms and clinical signs triggered by arbovirus infections, were analyzed using a recently developed, and in-house validated, 4-plex RT-qPCR TaqMan method for simultaneous detection and discrimination of the Zika and Chikungunya viruses. Subsequently, statistical analyses were performed in order to potentially establish recommendations regarding the choice of samples type to use for an efficient and early stage Zika infection diagnosis. On this basis, the use of only urine samples presented the highest probability to detect viral RNA from Zika virus. Moreover, such a probability was improved using both urine and saliva samples. Consequently, the added value of non-invasive samples, associated with a higher acceptance level for collection among patients, instead of serum samples, for the detection of Zika infections was illustrated.
寨卡病毒是一种引发重大疫情的虫媒病毒，可导致严重的健康问题，如神经疾病。在本研究中，于2015年至2016年在新喀里多尼亚和法属圭亚那采集了53例出现虫媒病毒感染引发的症状和临床体征患者的不同类型样本（血清、唾液和尿液），使用一种最近开发并经内部验证的四重RT-qPCR TaqMan方法进行分析，以同时检测和区分寨卡病毒和基孔肯雅病毒。随后进行了统计分析，以便有可能就选择用于高效早期寨卡病毒感染诊断的样本类型制定建议。在此基础上，仅使用尿液样本检测寨卡病毒RNA的概率最高。此外，同时使用尿液和唾液样本可提高这种概率。因此，说明了非侵入性样本在检测寨卡病毒感染方面的附加价值，与血清样本相比，患者对其采集的接受度更高。
