微小 RNA-181b 作为循环生物标志物调节心力衰竭中的炎症反应。
MicroRNA-181b Serves as a Circulating Biomarker and Regulates Inflammation in Heart Failure.
机构信息
Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, 37 Yiyuan Street Harbin, Heilongjiang, China 150001.
Department of Cardiology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin, Heilongjiang, China 150001.
出版信息
Dis Markers. 2021 Jul 1;2021:4572282. doi: 10.1155/2021/4572282. eCollection 2021.
Heart failure (HF) is the typical terminal stage of cardiac diseases involving inflammatory states. The function of microRNAs (miRNAs) in the progress of HF remains poorly understood. In this study, real-time PCR results showed a decreased expression of miRNA-181b (miR-181b) in HF patients compared with healthy individuals. Besides, miR-181b expressions were negatively correlated with hypersensitive C-reactive protein (hsCRP) levels in the serum of HF patients. Receiver operator characteristic (ROC) curve analysis showed that miR-181b was a diagnostic predictor of HF, and the area under the curve was 0.970 (DCM-induced HF group) and 0.962 (ICM-induced HF group). Strikingly, in HF rats induced by isoproterenol (ISO), the expression of miR-181b of heart tissue was suppressed before tumor necrosis factor-alpha (TNF-), interleukin-1 (IL-1), and interleukin-6 (IL-6) increase, as revealed by western blot and real-time PCR. Besides, the overexpression of miR-181b also decreased the expression of TNF-, IL-1, and IL-6 in lipopolysaccharide- (LPS-) induced neonatal cardiomyocytes. In conclusion, our results revealed that miR-181b might be a potential biomarker for HF and provided a novel target for anti-inflammatory therapy.
心力衰竭（HF）是涉及炎症状态的心脏疾病的典型终末期。miRNAs（miR-181b）在 HF 进展中的功能仍知之甚少。在这项研究中，实时 PCR 结果显示 HF 患者的 miR-181b 表达水平低于健康个体。此外，HF 患者血清中 miR-181b 的表达与超敏 C 反应蛋白（hsCRP）水平呈负相关。受试者工作特征（ROC）曲线分析表明，miR-181b 是 HF 的诊断预测因子，曲线下面积为 0.970（DCM 诱导的 HF 组）和 0.962（ICM 诱导的 HF 组）。引人注目的是，在异丙肾上腺素（ISO）诱导的 HF 大鼠中，Western blot 和实时 PCR 显示 miR-181b 的表达在肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）和白细胞介素-6（IL-6）增加之前就被抑制。此外，miR-181b 的过表达也降低了脂多糖（LPS）诱导的新生心肌细胞中 TNF-α、IL-1 和 IL-6 的表达。总之，我们的结果表明，miR-181b 可能是 HF 的潜在生物标志物，并为抗炎治疗提供了新的靶点。
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