Beukert Ulrike, Pfeiffer Nina, Ebmeyer Erhard, Hinterberger Valentin, Lueck Stefanie, Serfling Albrecht, Ordon Frank, Schulthess Albert Wilhelm, Reif Jochen Christoph
Institute for Resistance Research and Stress Tolerance, Julius Kuehn-Institute (JKI), 06484 Quedlinburg, Germany.
KWS LOCHOW GmbH, 29303 Bergen, Germany.
Biology (Basel). 2021 Jul 6;10(7):628. doi: 10.3390/biology10070628.
Leaf rust resistance is of high importance for a sustainable European wheat production. The expression of known resistance genes starts at different developmental stages of wheat. Breeding for resistance can be supported by a fast, precise, and resource-saving phenotyping. The examination of detached leaf assays of juvenile plants inoculated under controlled conditions and phenotyped by a robotic- and computer-based, high-throughput system is a promising approach in this respect. Within this study, the validation of the phenotyping workflow was conducted based on a winter wheat set derived from Central Europe and examined at different plant developmental stages. Moderate Pearson correlations of 0.38-0.45 comparing leaf rust resistance of juvenile and adult plants were calculated and may be mainly due to different environmental conditions. Specially, the infection under controlled conditions was limited by the application of a single rust race at only one time point. Our results suggest that the diversification with respect to the applied rust race spectrum is promising to increase the consistency of detached leaf assays and the transferability of its results to the field.
叶锈病抗性对于欧洲小麦的可持续生产至关重要。已知抗性基因的表达在小麦的不同发育阶段开始。快速、精确且节省资源的表型分析可为抗性育种提供支持。在受控条件下对幼苗进行离体叶片测定，并通过基于机器人和计算机的高通量系统进行表型分析，在这方面是一种很有前景的方法。在本研究中，基于源自中欧的冬小麦品种进行了表型分析工作流程的验证，并在不同的植物发育阶段进行了检测。计算得出幼苗和成株叶锈病抗性之间的皮尔逊相关性适中，为0.38 - 0.45，这可能主要是由于不同的环境条件。特别是，在受控条件下的感染受到仅在一个时间点应用单一锈菌小种的限制。我们的结果表明，在所应用的锈菌小种谱方面实现多样化有望提高离体叶片测定的一致性及其结果向田间的可转移性。
