Chen Yuan Yao, Zhao Xin, Moeder Wolfgang, Tun Hein M, Simons Elinor, Mandhane Piushkumar J, Moraes Theo J, Turvey Stuart E, Subbarao Padmaja, Scott James A, Kozyrskyj Anita L
Department of Pediatrics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 1C9, Canada.
Dalla Lana School of Public Health, University of Toronto, Toronto, ON M5T 1R4, Canada.
Microorganisms. 2021 Aug 31;9(9):1847. doi: 10.3390/microorganisms9091847.
Few studies consider the joint effect of multiple factors related to birth, delivery mode, intrapartum antibiotic prophylaxis and the onset of labour, on the abundance of and the quantity of this genus and its species subsp. in the infant gut microbiota. We implemented such a study.
Among 1654 Canadian full-term infants, the gut microbiota of faecal samples collected at 3 months were profiled by 16S rRNA sequencing; the genus and subsp. were quantified by qPCR. Associations between and other gut microbiota were examined by Spearman's rank correlation.
Following vaginal birth, maternal IAP exposure was associated with reduced absolute quantities of bifidobacteria among vaginally delivered infants (6.80 vs. 7.14 log (gene-copies/g faeces), < 0.05), as well as their lowered abundance relative to other gut microbiota. IAP differences in infant gut bifidobacterial quantity were independent of maternal pre-pregnancy body-mass-index (BMI), and remarkably, they were limited to breastfed infants. Pre-pregnancy BMI adjustment revealed negative associations between absolute quantities of bifidobacteria and CS with or without labour in non-breastfed infants, and CS with labour in exclusively breastfed infants. Significant correlations between abundance and other microbial taxa were observed.
This study documented the impact of the birth mode and feeding status on the abundance of gut , and pointed to the important ecological role of the genus in gut microbiota due to its strong interaction with other gut microbiota in early infancy.
很少有研究考虑与出生、分娩方式、产时抗生素预防和分娩发动相关的多种因素对婴儿肠道微生物群中该属及其亚种的丰度和数量的联合影响。我们开展了这样一项研究。
在1654名加拿大足月婴儿中，通过16S rRNA测序对3个月时采集的粪便样本的肠道微生物群进行分析；通过qPCR对该属及其亚种进行定量。通过Spearman等级相关性检验该属与其他肠道微生物群之间的关联。
经阴道分娩后，母亲暴露于产时抗生素预防与经阴道分娩婴儿中双歧杆菌的绝对数量减少有关（6.80对7.14 log（基因拷贝数/克粪便），P<0.05），以及相对于其他肠道微生物群其丰度降低。婴儿肠道双歧杆菌数量的产时抗生素预防差异与母亲孕前体重指数（BMI）无关，而且，这种差异仅限于母乳喂养的婴儿。孕前BMI调整显示，在非母乳喂养婴儿中，无论是否分娩，双歧杆菌的绝对数量与剖宫产呈负相关，在纯母乳喂养婴儿中，双歧杆菌的绝对数量与分娩时剖宫产呈负相关。观察到该属丰度与其他微生物分类群之间存在显著相关性。
本研究记录了分娩方式和喂养状态对肠道该属丰度的影响，并指出该属在肠道微生物群中具有重要的生态作用，因为它在婴儿早期与其他肠道微生物群有强烈的相互作用。
