State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Int J Mol Sci. 2019 Jul 5;20(13):3306. doi: 10.3390/ijms20133306.
Our objective was to investigate the effects of different delivery and feeding modes on the gut microbiota composition of early infants with special emphasis on and profiles at species level. 16S rRNA V3-V4 regions, bifidobacterial, and lactobacilli genes from infant feces were sequenced by Illumina MiSeq. Gut microbiota abundance was significantly different, where standard vaginally delivered (SVD) and breast-fed (BF) groups were higher in comparison with caesarean section (CS), milk-powder-fed (MPF), and mixed-fed (MF) groups. The genus unclassified Enterobacteriaceae was dominant, followed by , which was highly abundant in SVD and BF groups. The dominant species in all groups were , and . and the diversity of in SVD and BF groups were significantly higher. For profiles, and were dominant among all the groups, while species in CS and MPF groups were more diverse. Functional predictions showed significant differences between delivery mode and feeding groups, such as phosphotransferase system as well as taurine and hypotaurine metabolism. In early infants with different delivery and feeding methods, gut microbiota-particularly bifidobacteria and lactobacilli communities-showed significant differences, with strong implications for physiological functions.
我们的目的是研究不同分娩和喂养方式对早期婴儿肠道微生物群落组成的影响，特别关注和的特征在物种水平上。通过 Illumina MiSeq 对婴儿粪便中的 16S rRNA V3-V4 区、双歧杆菌和乳杆菌基因进行测序。肠道微生物群落丰度存在显著差异，与剖宫产（CS）、奶粉喂养（MPF）和混合喂养（MF）组相比，标准阴道分娩（SVD）和母乳喂养（BF）组的丰度更高。未分类肠杆菌科属占优势，其次是，在 SVD 和 BF 组中高度丰富。所有组中占优势的 物种是 、 和 。SVD 和 BF 组中的 和多样性显著更高。对于 谱，在所有组中均占优势，而 CS 和 MPF 组中的 物种则更为多样化。功能预测显示，分娩方式和喂养组之间存在显著差异，如磷酸转移酶系统以及牛磺酸和次牛磺酸代谢。在具有不同分娩和喂养方式的早期婴儿中，肠道微生物群落——特别是双歧杆菌和乳杆菌群落——存在显著差异，对生理功能有重要影响。
