Department of Epidemiology, The Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Center for Molecular Epidemiology, The Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
BJOG. 2020 Jan;127(2):217-227. doi: 10.1111/1471-0528.15799. Epub 2019 Jun 5.
To evaluate the potential impact of intrapartum antibiotics, and their specific classes, on the infant gut microbiota in the first year of life.
Prospective study of infants in the New Hampshire Birth Cohort Study (NHBCS).
Rural New Hampshire, USA.
Two hundred and sixty-six full-term infants from the NHBCS.
Intrapartum antibiotic use during labour and delivery was abstracted from medical records. Faecal samples collected at 6 weeks and 1 year of age were characterised by 16S rRNA sequencing, and metagenomics analysis in a subset of samples.
Maternal exposure to antibiotics during labour and delivery.
Taxonomic and functional profiles of faecal samples.
Infant exposure to intrapartum antibiotics, particularly to two or more antibiotic classes, was independently associated with lower microbial diversity scores as well as a unique bacterial community at 6 weeks (GUnifrac, P = 0.02). At 1 year, infants in the penicillin-only group had significantly lower α diversity scores than infants not exposed to intrapartum antibiotics. Within the first year of life, intrapartum exposure to penicillins was related to a significantly lower increase in several taxa including Bacteroides, use of cephalosporins was associated with a significantly lower rise over time in Bifidobacterium and infants in the multi-class group experienced a significantly higher increase in Veillonella dispar.
Our findings suggest that intrapartum antibiotics alter the developmental trajectory of the infant gut microbiome, and specific antibiotic types may impact community composition, diversity and keystone immune training taxa.
Class of intrapartum antibiotics administered during delivery relates to maturation of infant gut microbiota.
评估分娩期抗生素及其特定类别对婴儿肠道微生物群在生命第一年的潜在影响。
新罕布什尔州出生队列研究（NHBCS）中的婴儿前瞻性研究。
美国新罕布什尔州农村。
来自 NHBCS 的 266 名足月婴儿。
从病历中提取分娩期间使用的产时抗生素。在 6 周和 1 岁时收集粪便样本，通过 16S rRNA 测序和部分样本的宏基因组学分析进行特征描述。
母亲在分娩期间接触抗生素。
粪便样本的分类和功能谱。
婴儿暴露于产时抗生素，特别是两种或更多种抗生素类别，与微生物多样性评分较低以及 6 周时独特的细菌群落独立相关（GUnifrac，P=0.02）。在 1 岁时，青霉素组婴儿的 α 多样性评分明显低于未暴露于产时抗生素的婴儿。在生命的第一年，产时接触青霉素与包括拟杆菌在内的几个分类群的显著降低有关，使用头孢菌素与双歧杆菌的时间推移呈显著降低有关，多类组婴儿经历了明显更高的差异韦荣球菌增加。
我们的研究结果表明，产时抗生素改变了婴儿肠道微生物群的发育轨迹，特定的抗生素类型可能影响群落组成、多样性和关键免疫训练分类群。
分娩期间给予的抗生素类别与婴儿肠道微生物群的成熟有关。
