Natsume Yukie, Yamada Takako, Iida Tetsuo, Ozaki Nobuaki, Gou Yang, Oshida Yoshiharu, Koike Teruhiko
Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya, Aichi, Japan.
Research and Development, Matsutani Chemical Industry Co., Ltd., Itami, Hyogo, Japan.
Heliyon. 2021 Sep 17;7(9):e08013. doi: 10.1016/j.heliyon.2021.e08013. eCollection 2021 Sep.
d-Allulose, a C-3 epimer of d-fructose, is a rare sugar that has no calories. Although d-allulose has been reported to have several health benefits, such as anti-obesity and anti-diabetic effects, there have been no reports evaluating the effects of d-allulose on insulin resistance using a hyperinsulinemic-euglycemic clamp (HE-clamp). Therefore, we investigated the effects of d-allulose on a high-sucrose diet (HSD)-induced insulin resistance model. Wistar rats were randomly divided into three dietary groups: HSD containing 5% cellulose (HSC), 5% d-allulose (HSA), and a commercial diet. The insulin tolerance test (ITT) and HE-clamp were performed after administration of the diets for 4 and 7 weeks. After 7 weeks, the muscle and adipose tissues of rats were obtained to analyze Akt signaling via western blotting, and plasma adipocytokine levels were measured. ITT revealed that d-allulose ameliorated systemic insulin resistance. Furthermore, the results of the 2-step HE-clamp procedure indicated that d-allulose reversed systemic and muscular insulin resistance. d-Allulose reversed the insulin-induced suppression of Akt phosphorylation in the soleus muscle and epididymal fat tissues and reduced plasma TNF-α levels. This study is the first to show that d-allulose improves systemic and muscle insulin sensitivity in conscious rats.
D-阿洛酮糖是D-果糖的C-3差向异构体，是一种无热量的稀有糖。尽管已有报道称D-阿洛酮糖具有多种健康益处，如抗肥胖和抗糖尿病作用，但尚无使用高胰岛素-正葡萄糖钳夹技术（HE钳夹）评估D-阿洛酮糖对胰岛素抵抗影响的报道。因此，我们研究了D-阿洛酮糖对高蔗糖饮食（HSD）诱导的胰岛素抵抗模型的影响。将Wistar大鼠随机分为三个饮食组：含5%纤维素的HSD（HSC）组、含5%D-阿洛酮糖的HSD（HSA）组和商业饮食组。在给予饮食4周和7周后进行胰岛素耐量试验（ITT）和HE钳夹。7周后，获取大鼠的肌肉和脂肪组织，通过蛋白质印迹法分析Akt信号通路，并测量血浆脂肪细胞因子水平。ITT显示D-阿洛酮糖改善了全身胰岛素抵抗。此外，两步HE钳夹程序的结果表明D-阿洛酮糖逆转了全身和肌肉胰岛素抵抗。D-阿洛酮糖逆转了胰岛素诱导的比目鱼肌和附睾脂肪组织中Akt磷酸化的抑制，并降低了血浆TNF-α水平。本研究首次表明D-阿洛酮糖可改善清醒大鼠的全身和肌肉胰岛素敏感性。
