Johnson B C, Gajjar A, Kubo C, Good R A
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5659-62. doi: 10.1073/pnas.83.15.5659.
Autoimmunity-prone (NZB x NZW)F1 (B/W) female mice are used as a model of human lupus erythematosus. When full-fed, these mice die of glomerulonephritis between 7 and 11 (average 9) months of age. When food intake is restricted to 60% of calories, the onset of this disease is delayed and the mice live greatly prolonged lives free of disease. Since high protein intake is commonly associated with acceleration of kidney damage in humans and experimental animals, the current experiments were designed to employ diets in which protein concentration was as high as possible. The observations demonstrate clearly that with this model of autoimmune disease, total calorie intake (from whatever source) exerts an overriding influence on life span. A higher calorie intake leads to early death and restricted-calorie intake leads to an increased life span. When B/W mice are full-fed, with respect to calories, feeding diets of greatly differing protein composition did not influence life span significantly. By contrast, calorie restriction of diets, even of very high protein content or of lower protein content, greatly prolonged life of B/W mice. Even with exceedingly high protein intake (greater than 83% of the calories) it is not protein per se but the total calorie intake that exerts the greatest influence that determines length of life in mice of this autoimmunity- and glomerulonephritis-prone strain.
易患自身免疫性疾病的（新西兰黑鼠×新西兰白鼠）F1（B/W）雌性小鼠被用作人类红斑狼疮的模型。正常进食时，这些小鼠在7至11（平均9）月龄时死于肾小球肾炎。当食物摄入量限制在热量的60%时，这种疾病的发病会延迟，并且小鼠的寿命会大大延长且无疾病。由于高蛋白摄入在人类和实验动物中通常与肾脏损伤加速相关，当前的实验旨在采用蛋白质浓度尽可能高的饮食。观察结果清楚地表明，对于这种自身免疫性疾病模型，总热量摄入（无论来源如何）对寿命产生首要影响。较高的热量摄入导致早期死亡，而热量摄入受限则导致寿命延长。当B/W小鼠正常进食时，就热量而言，喂食蛋白质组成差异很大的饮食对寿命没有显著影响。相比之下，饮食的热量限制，即使是高蛋白含量或低蛋白含量的饮食，也能大大延长B/W小鼠的寿命。即使蛋白质摄入量极高（超过热量的83%），对这种易患自身免疫性疾病和肾小球肾炎的品系小鼠来说，对寿命起最大影响作用的不是蛋白质本身，而是总热量摄入。
