Chai Min Hian, Sukiman Muhammad Zikree, Najib Nurlailasari Mohammad, Mohabbar Nor Arifah, Azizan Nur Aina Nadhirah Mohd, Mohamad Noor Muzamil, Ariffin Siti Mariam Zainal, Ghazali Mohd Faizal
School of Animal Science, Aquatic Science and Environment, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut, Terengganu, Malaysia.
Centralised Laboratory Management Centre, Universiti Sultan Zainal Abidin, Besut, Terengganu, Malaysia.
J Adv Vet Anim Res. 2021 Sep 19;8(3):388-395. doi: 10.5455/javar.2021.h527. eCollection 2021 Sep.
This study aims to investigate the prevalence and antibiogram of and methicillin-resistance (MRSA) in rabbits, rabbit handlers, and rabbitry environments in Terengganu.
Swab samples from 183 rabbits (183 oral and 183 ear swabs), 45 rabbit handlers (45 oral and 45 nasal), and environmental ( = 180) samples from rabbitries were collected from 10 rabbit farms in Terengganu. The associated isolates from the swabs were isolated using phenotypic microbiology tests. The bacteria were confirmed by polymerase chain reaction targeting () and (MRSA) genes. The antibiogram of all isolates was determined using the Kirby-Bauer test.
was detected in 19% of rabbits, 26.7% of rabbit handlers, and 8.8% of swabs from the rabbitry environment. However, MRSA (0%) could not be detected. Antibiotic susceptibility test revealed that from rabbits showed low resistance (<20%) against 15 different antibiotics while fully susceptible to 4 antibiotics. Meanwhile, from rabbit handlers showed high resistance against penicillin (86%), oxacillin (64%), and amoxicillin (50%).
This study suggests the emergence of antibiotic-resistant in rabbit farms settings. Therefore, careful selection of antimicrobial agents will be essential to preserve the effectiveness of treatments toward infections.
本研究旨在调查登嘉楼州家兔、家兔饲养者及养兔场环境中金黄色葡萄球菌的流行情况及抗菌谱，以及耐甲氧西林金黄色葡萄球菌（MRSA）的情况。
从登嘉楼州的10个养兔场收集了183只家兔的拭子样本（183份口腔拭子和183份耳部拭子）、45名家兔饲养者的拭子样本（45份口腔拭子和45份鼻腔拭子）以及养兔场的环境样本（n = 180）。使用表型微生物学试验从拭子中分离出相关的金黄色葡萄球菌菌株。通过针对金黄色葡萄球菌（SA）和耐甲氧西林金黄色葡萄球菌（MRSA）基因的聚合酶链反应确认细菌。使用 Kirby-Bauer 试验确定所有金黄色葡萄球菌菌株的抗菌谱。
在19%的家兔、26.7%的家兔饲养者以及8.8%的养兔场环境拭子中检测到金黄色葡萄球菌。然而，未检测到耐甲氧西林金黄色葡萄球菌（0%）。抗生素敏感性试验显示，家兔来源的金黄色葡萄球菌对15种不同抗生素的耐药率较低（<20%），而对4种抗生素完全敏感。同时，家兔饲养者来源的金黄色葡萄球菌对青霉素（86%）、苯唑西林（64%）和阿莫西林（50%）耐药率较高。
本研究表明养兔场环境中出现了耐抗生素的金黄色葡萄球菌。因此，谨慎选择抗菌药物对于保持治疗金黄色葡萄球菌感染的有效性至关重要。
