Tien Nguyen Quang Duc, Ma Xiao, Man Le Quang, Chi Duong Thi Kim, Huy Nguyen Xuan, Nhut Duong-Tan, Rombauts Stephane, Ut Tran, Loc Nguyen Hoang
Bioactive Compound Institute, University of Sciences, Hue University, Hue, 530000 Vietnam.
Department of Biology, Bioactive Compound Institute, University of Sciences, Hue University, Hue, 530000 Vietnam.
Physiol Mol Biol Plants. 2021 Oct;27(10):2215-2229. doi: 10.1007/s12298-021-01076-1. Epub 2021 Oct 11.
Vietnamese ginseng ( Ha Grushv.), also known as Ngoc Linh ginseng, is a high-value herb in Vietnam. Vietnamese ginseng has been proven to be effective in enhancing the immune system, human memory, anti-stress, anti-inflammatory, anti-cancer, and prevent aging. The present study reports the first draft whole-genome of Vietnamese ginseng and the identification of potential genes involved in the triterpenoid metabolic pathway. De novo whole-genome assembly was performed successfully from a data of approximately 139 Gbps of 394,802,120 high quality reads to generate 9815 scaffolds with an N50 value of 572,722 bp from the leaf of Vietnamese ginseng. The assembled genome of Vietnamese ginseng is 3,001,967,204 bp long containing 79,374 gene models. Among them, there are 55,012 genes (69.30%) were annotated by various public molecular biology databases. The potential genes involved in triterpenoid saponin biosynthesis in Vietnamese ginseng and their metabolic pathway were also predicted." Three genes encoding squalene monooxygenase isozymes in Vietnamese ginseng were cloned, sequenced and characterized. Moreover, expression levels of several key genes involved in terpenoid biosynthesis in different parts of Vietnamese ginseng were also analyzed. The SSR markers were detected by various programs from both of assembly full dataset of Vietnamese ginseng genome and predicted genes. The present work provided important data of the draft whole-genome of Vietnamese ginseng for further studies to understand the role of genes involved in ginsenoside biosynthesis and their metabolic pathway at the molecular level of this rare medicinal species.
The online version contains supplementary material available at 10.1007/s12298-021-01076-1.
越南人参（Ha Grushv.），也被称为野山参，是越南一种具有高价值的草药。越南人参已被证明在增强免疫系统、提高人类记忆力、抗应激、抗炎、抗癌和预防衰老方面有效。本研究报告了越南人参的首个全基因组草图以及参与三萜代谢途径的潜在基因的鉴定。利用来自越南人参叶片的约139 Gbps的394,802,120条高质量 reads数据成功进行了从头全基因组组装，生成了9815个支架，N50值为572,722 bp。越南人参组装后的基因组长度为3,001,967,204 bp，包含79,374个基因模型。其中，有55,012个基因（69.30%）被各种公共分子生物学数据库注释。还预测了越南人参中参与三萜皂苷生物合成的潜在基因及其代谢途径。克隆、测序并鉴定了越南人参中编码鲨烯单加氧酶同工酶的三个基因。此外，还分析了越南人参不同部位参与萜类生物合成的几个关键基因的表达水平。通过各种程序从越南人参基因组的组装完整数据集和预测基因中检测到了SSR标记。本研究为进一步了解这种珍稀药用植物在分子水平上人参皂苷生物合成相关基因及其代谢途径的作用提供了越南人参全基因组草图的重要数据。
在线版本包含可在10.1007/s12298-021-01076-1获取的补充材料。
