Department of Chemistry, University of Nebraska-Lincoln, United States; The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, United States.
Department of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, United States; The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, United States.
J Mol Biol. 2022 Apr 30;434(8):167346. doi: 10.1016/j.jmb.2021.167346. Epub 2021 Nov 8.
Noncanonical amino acid mutagenesis has emerged as a powerful tool for the study of protein structure and function. While triplet nonsense codons, especially the amber codon, have been widely employed, quadruplet codons have attracted attention for the potential of creating additional blank codons for noncanonical amino acids mutagenesis. In this review, we discuss methodologies and applications of quadruplet codon decoding in genetic code expansion both in vitro and in vivo.
非天然氨基酸突变已成为研究蛋白质结构和功能的有力工具。虽然三联体无义密码子，尤其是琥珀密码子，已被广泛应用，但四联体密码子因其有可能为非天然氨基酸突变创造额外的空白密码子而受到关注。本文综述了体外和体内遗传密码扩展中四联体密码子解码的方法学和应用。
