Huang Hao, Yuan Guotao, Xu Ying, Gao Yuan, Mao Qiulian, Zhang Yin, Bai Lu, Li Weijie, Wu Anqing, Hu Wentao, Pan Yue, Zhou Guangming
State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, PR China.
Bioact Mater. 2021 Jul 28;9:157-167. doi: 10.1016/j.bioactmat.2021.07.025. eCollection 2022 Mar.
The integration of photothermal therapy (PTT) with gene therapy (GT) in a single nanoscale platform demonstrates great potential in cancer therapy. Porous iron oxide nanoagents (PIONs) are widely used as magnetic nanoagents in the drug delivery field and also serve as a photothermal nanoagent for photothermal therapy. However, the therapeutic efficacy of PIONs-mediated GT has not been studied. The long noncoding RNA (lncRNA) CRYBG3 (LNC CRYBG3), a lncRNA induced by heavy ion irradiation in lung cancer cells, has been reported to directly bind to globular actin (G-actin) and cause degradation of cytoskeleton and blocking of cytokinesis, thus indicating its potential for use in GT by simulating the effect of heavy ion irradiation and functioning as an antitumor drug. In the present study, we investigated the possibility of combining PIONs-mediated PTT and LNC CRYBG3-mediated GT to destroy non-small cell lung cancer (NSCLC) cells both and . The combination therapy showed a high cancer cell killing efficacy, and the cure rate was better than that achieved using PTT or GT alone. Moreover, as a type of magnetic nanoagent, PIONs can be used for magnetic resonance imaging (MRI) and photoacoustic imaging (PAI) both and . These findings indicate that the new combination therapy has high potential for cancer treatment.
在单一纳米级平台上将光热疗法（PTT）与基因疗法（GT）相结合，在癌症治疗中显示出巨大潜力。多孔氧化铁纳米剂（PIONs）在药物递送领域被广泛用作磁性纳米剂，同时也用作光热疗法的光热纳米剂。然而，PIONs介导的基因疗法的治疗效果尚未得到研究。长链非编码RNA（lncRNA）CRYBG3（LNC CRYBG3）是一种在肺癌细胞中由重离子辐射诱导产生的lncRNA，据报道它能直接结合球状肌动蛋白（G-肌动蛋白），导致细胞骨架降解和胞质分裂受阻，因此通过模拟重离子辐射的作用并作为抗肿瘤药物，显示出其在基因疗法中的应用潜力。在本研究中，我们研究了将PIONs介导的PTT与LNC CRYBG3介导的GT相结合来体内外破坏非小细胞肺癌（NSCLC）细胞的可能性。联合疗法显示出高癌细胞杀伤效果，治愈率优于单独使用PTT或GT。此外，作为一种磁性纳米剂，PIONs在体内外均可用于磁共振成像（MRI）和光声成像（PAI）。这些发现表明，这种新的联合疗法在癌症治疗方面具有很高的潜力。
