Tsiogkas Sotirios G, Mavropoulos Αthanasios, Skyvalidas Dimitrios N, Patrikiou Eleni, Ntavari Niki, Daponte Athina Ioanna, Grammatikopoulou Maria G, Dardiotis Efthimios, Roussaki-Schulze Aggeliki-Victoria, Sakkas Lazaros I, Zafiriou Efterpi, Bogdanos Dimitrios P
Department of Rheumatology and Clinical Immunology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.
Department of Dermatology, Faculty of Medicine, School of Health Sciences, University of Thessaly, Larissa, Greece.
Immunol Res. 2022 Apr;70(2):161-173. doi: 10.1007/s12026-021-09251-y. Epub 2021 Nov 25.
The anthocyanidin delphinidin reduces psoriasiform lesions and inflammatory mediators in human cell culture systems. Its role in psoriatic disease has not yet been investigated. We assessed delphinidin's in vitro immunomodulatory effect on ex vivo stimulated peripheral blood mononuclear cells (PBMCs) from 50 individuals [26 with psoriasis, 10 with psoriatic arthritis (PsA) and 14 healthy controls (HCs)]. Cells were either left untreated or stimulated with PMA plus ionomycin in the presence or absence of delphinidin. Intracellular production of interferon-γ (IFNγ), interleukin-17A (IL-17A), and interleukin-10 (IL-10) was measured flow cytometrically. Delphinidin dose-dependently reduced IFNγ T cells from patients and HCs. The mean IFNγ decrease in CD4 T subpopulations was 42.5 ± 28% for psoriasis patients, 51.8 ± 21.5% for PsA patients and 49 ± 17% for HCs (p < 0.001 for all). Similarly, IFNγ reduction in CD8 T cells was 34 ± 21.6% for psoriasis patients, 47.1 ± 22.8% for PsA and 44.8 ± 14.3% for HCs (P < 0.001 for all). An inhibitory effect of delphinidin was also noted in IFNγ producing NKs and NKTs from psoriasis individuals. Delphinidin also significantly decreased IL-17 CD4 T cells in all tested subjects, with marginal effect on the increase of IL-10-producing T regulatory subsets. In conclusion, delphinidin exerts a profound in vitro anti-inflammatory effect in psoriasis and psoriatic arthritis by inhibiting IFNγ innate and adaptive cells and T helper (Th) 17 cells. If this effect is also exerted in vivo, delphinidin may be regarded as a nutraceutical with immunosuppressive potential.
花青素飞燕草素可减少人类细胞培养系统中的银屑病样病变和炎症介质。其在银屑病疾病中的作用尚未得到研究。我们评估了飞燕草素对来自50名个体（26名银屑病患者、10名银屑病关节炎患者和14名健康对照者）的体外刺激外周血单个核细胞（PBMC）的免疫调节作用。细胞要么不进行处理，要么在有或没有飞燕草素的情况下用佛波酯（PMA）加离子霉素进行刺激。通过流式细胞术测量细胞内γ干扰素（IFNγ）、白细胞介素-17A（IL-17A）和白细胞介素-10（IL-10）的产生。飞燕草素剂量依赖性地减少了患者和健康对照者的IFNγ T细胞。银屑病患者CD4 T亚群中IFNγ的平均减少率为42.5±28%，银屑病关节炎患者为51.8±21.5%，健康对照者为49±17%（所有p<0.001）。同样，银屑病患者CD8 T细胞中IFNγ的减少率为34±21.6%，银屑病关节炎患者为47.1±22.8%，健康对照者为44.8±14.3%（所有P<0.001）。在银屑病个体产生IFNγ的自然杀伤细胞（NK）和自然杀伤T细胞（NKT）中也观察到了飞燕草素的抑制作用。飞燕草素还显著降低了所有受试对象中产生IL-17的CD4 T细胞，对产生IL-10的调节性T细胞亚群的增加影响较小。总之，飞燕草素通过抑制IFNγ天然和适应性细胞以及辅助性T（Th）17细胞，在体外对银屑病和银屑病关节炎发挥了显著的抗炎作用。如果这种作用在体内也能发挥，飞燕草素可能被视为一种具有免疫抑制潜力的营养保健品。
