Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education, Beijing Advanced Innovation Centre for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, No.37 Xueyuan Road, Beijing, 100191, China.
International Joint Research Center of Aerospace Biotechnology & Medical Engineering, Beihang University, Beijing, 100191, China.
Appl Microbiol Biotechnol. 2022 Jan;106(1):441-453. doi: 10.1007/s00253-021-11698-w. Epub 2021 Dec 6.
As the core food crop of a bioregenerative life support system (BLSS), wheat is susceptible to pathogen infection due to the lack of effective microbial communities in the confined and isolated environment. Therefore, a thorough understanding of the dynamic changes in wheat rhizosphere fungi is of great significance for improving wheat production and ensuring the stability of the BLSS. In the current study, we collected samples of rhizosphere fungi in the four growth stages of wheat grown in the "Lunar Palace 365" experiment. We employed bioinformatics methods to analyze the samples' species composition characteristics, community network characteristics, and FUNGuild function analysis. We found that the species composition of rhizosphere fungi in the wheat at the tillering stage changed greatly in the closed and isolated environment, while the species composition in the seedling, flowering, and mature stage were relatively stable. The results of the FUNGuild function analysis showed that the functions of rhizosphere fungi changed during wheat development. The rhizosphere fungal community was centered on Ascomycota, Mortierellomycota, and Chytridiomycota, and the community showed the characteristics of a "small world" arrangement. The stage of wheat seedlings is characterized by a greater abundance, diversity, and complexity of the network of interactions in the rhizosphere mycorrhiza community, while the tillering stage exhibited a greater clustering coefficient. Based on the changes in species composition, guild function regulation, and community structure differences of the wheat rhizosphere fungi in the BLSS, our study identified the critical fungal species during wheat development, providing a reference for ensuring the health and yield of plants in the BLSS system. KEY POINTS: • The diversity, composition, FUNguild, and network structure of rhizosphere fungi were analyzed. • Ascomycota, Mortierellomycota, and Chytridiomycota were the center of the rhizosphere fungal community network. • The effects of different wheat developmental stages on the community composition, function, and network structure of rhizosphere fungi were examined.
作为生物再生生命支持系统（BLSS）的核心粮食作物，小麦由于在封闭和隔离的环境中缺乏有效的微生物群落，容易受到病原体的感染。因此，深入了解小麦根际真菌的动态变化对于提高小麦产量和确保 BLSS 的稳定性具有重要意义。在本研究中，我们采集了“月宫 365”实验中四个生长阶段的小麦根际真菌样本。我们采用生物信息学方法分析了样本的物种组成特征、群落网络特征和 FUNGuild 功能分析。我们发现，在封闭和隔离的环境中，小麦分蘖期根际真菌的物种组成变化很大，而幼苗期、开花期和成熟期的物种组成相对稳定。FUNGuild 功能分析结果表明，根际真菌的功能在小麦发育过程中发生了变化。根际真菌群落以子囊菌门、毛霉门和壶菌门为主，群落呈现出“小世界”排列的特征。小麦幼苗期的根际共生真菌网络具有更大的丰度、多样性和复杂性，而分蘖期则表现出更大的聚类系数。基于 BLSS 中小麦根际真菌的物种组成、菌属功能调控和群落结构差异的变化，我们确定了小麦发育过程中的关键真菌物种，为确保 BLSS 系统中植物的健康和产量提供了参考。
分析了根际真菌的多样性、组成、FUNGuild 和网络结构。
子囊菌门、毛霉门和壶菌门是根际真菌群落网络的中心。
研究了不同小麦发育阶段对根际真菌群落组成、功能和网络结构的影响。
