Zhou He, Sun Yuqing, Li Xin, Zhou Ziyu, Ma Kexin, Guo Wenxuan, Liang Yuting, Xie Xingyi, Zhang Jingxian, Wang Qian, Liu Yang
Key Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Dalian 116023, China.
Key Laboratory of Marine Bio-Resources Sustainable Utilization in Liaoning Province's University, Dalian Ocean University, Dalian 116023, China.
Animals (Basel). 2021 Nov 30;11(12):3419. doi: 10.3390/ani11123419.
The phenotypic sex of fish is usually plastic. Low-temperature treatment induces the masculinization of , resulting in pseudo-males (PM) with the physiological sex of a male (M) and genetic sex of a female (F). For a comparison of gonadal transcriptomes, we collected gonads from three groups of (F, M, and PM) for high-throughput transcriptome sequencing. The results provided 467,640,218 raw reads (70.15 Gb) and a total of 436,151,088 clean reads (65.43 Gb), with an average length of 150 bp. Only 79 differentially expressed genes (DEGs) were identified between F and PM, whereas 12,041 and 11,528 DEGs were identified between F and M, and PM and M, respectively. According to the functional annotation of DEGs, 13 DEGs related to gonadal development were screened (, , , , , , , , , , , , and ) which might participate in formating PM. A quantitative PCR of the DEGs confirmed the reliability of the RNA-seq. Our results provide an important contribution to the genome sequence resources for and insight into the molecular mechanism of masculinization in a cultured fish subject to low-temperature treatment.
鱼类的表型性别通常具有可塑性。低温处理会诱导其雄性化，产生具有雄性生理性别和雌性遗传性别的伪雄鱼（PM）。为了比较性腺转录组，我们从三组鱼（F、M和PM）中收集性腺进行高通量转录组测序。结果共获得467,640,218条原始 reads（70.15 Gb），共436,151,088条clean reads（65.43 Gb），平均长度为150 bp。F和PM之间仅鉴定出79个差异表达基因（DEG），而F和M之间以及PM和M之间分别鉴定出12,041个和11,528个DEG。根据DEG的功能注释，筛选出13个与性腺发育相关的DEG（、、、、、、、、、、、、和），它们可能参与了伪雄鱼的形成。对这些DEG进行定量PCR验证了RNA测序的可靠性。我们的结果为该鱼类的基因组序列资源做出了重要贡献，并深入了解了低温处理养殖鱼类雄性化的分子机制。
