Department of Health Science and Interdisciplinary Research Center of Autoimmune Disease (IRCAD), Università del Piemonte Orientale, Via Solaroli, 17, 28100 Novara, Italy.
Department of Science and Technology Innovation, Università del Piemonte Orientale, Via T. Michel, 11, 15121 Alessandria, Italy.
Int J Mol Sci. 2021 Dec 14;22(24):13443. doi: 10.3390/ijms222413443.
Photodynamic therapy (PDT) has been pointed out as a candidate for improving melanoma treatment. Nanotechnology application in PDT has increased its efficacy by reducing side effects. Herein, mesoporous silica nanoparticles (MSNs) conjugated with verteporfin (Ver-MSNs), in use with PDT, were administered in mice to evaluate their efficacy on lymphoangiogenesis and micrometastasis in melanoma. Melanoma was induced in mice by the subcutaneous injection of B16-F10 cells. The mice were transcutaneously treated with MSNs, Ver-MSNs, or glycerol and exposed to red light. The treatment was carried out four times until day 20. Lymphangiogenesis and micrometastasis were identified by the immunohistochemical method. Lymphoangiogenesis was halved by MSN treatment compared with the control animals, whereas the Ver-MSN treatment almost abolished it. A similar reduction was also observed in lung micrometastasis. PDT with topically administrated Ver-MSNs reduced melanoma lymphoangiogenesis and lung micrometastasis, as well as tumor mass and angiogenesis, and therefore their use could be an innovative and useful tool in melanoma clinical therapy.
光动力疗法（PDT）被认为是改善黑色素瘤治疗的一种候选方法。纳米技术在 PDT 中的应用通过减少副作用提高了其疗效。在此，用与 PDT 联合使用的阿霉素偶联介孔硅纳米粒子（Ver-MSNs）对小鼠进行给药，以评估其在黑色素瘤淋巴管生成和微转移中的疗效。通过皮下注射 B16-F10 细胞在小鼠中诱导黑色素瘤。用 MSNs、Ver-MSNs 或甘油对小鼠进行经皮治疗，并暴露于红光下。治疗持续到第 20 天，共进行 4 次。通过免疫组织化学方法鉴定淋巴管生成和微转移。与对照组动物相比，MSN 治疗使淋巴管生成减少了一半，而 Ver-MSN 治疗几乎使其完全消除。在肺微转移中也观察到类似的减少。局部施用 Ver-MSNs 的 PDT 减少了黑色素瘤的淋巴管生成和肺微转移，以及肿瘤体积和血管生成，因此它们的使用可能是黑色素瘤临床治疗的一种创新且有用的工具。
