Department of Chemical Engineering, Faculty of Engineering, Arak University, Arak, Iran; Department of Tissue Engineering, TISSUEHUB Co., Tehran, Iran; Tissue Engineering Hub (TEHUB), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
Department of Chemistry, Shahreza Branch, Islamic Azad University, Shahreza, Isfahan, Iran.
Int J Biol Macromol. 2022 Apr 15;204:321-332. doi: 10.1016/j.ijbiomac.2022.02.019. Epub 2022 Feb 8.
Utilizing plant-based scaffolds has pulled in the consideration of tissue engineers. Plant tissues own different structures with particular porosity and structure. In this study, the stem of the Alstroemeria flower was designated for decellularization to fabricate a new scaffold. The stems were decellularized and called AFSP and then modified by chitosan and named AFSPC. Osteoblast precursor cell line was employed to assess the biological potential of the final scaffolds. The results uncovered that AFSP owns linear microchannels with a smooth surface. AFSPC delineated uniform chitosan coating on the walls with appropriate roughness. AFSPC showed higher potential in swelling, degradation, diffusion, and having a porous structure than AFSP. Modification with chitosan improved mechanical behavior. Biological assays depicted no cytotoxicity for AFSP and AFSPC. AFSPC showed good cell attachment, proliferation, and migration. In conclusion, modified tissue plants can be a good candidate for tissue engineering of both soft and hard tissues.
利用植物支架引起了组织工程师的关注。植物组织具有不同的结构，具有特定的孔隙率和结构。在这项研究中，将六出花的茎指定进行脱细胞化处理，以制造新的支架。将茎进行脱细胞化处理并称为 AFSP，然后用壳聚糖进行修饰并命名为 AFSPC。使用成骨前体细胞系评估最终支架的生物学潜力。结果表明，AFSP 具有线性微通道，表面光滑。AFSPC 在壁上描绘出均匀的壳聚糖涂层，具有适当的粗糙度。与 AFSP 相比，AFSPC 在溶胀、降解、扩散和具有多孔结构方面具有更高的潜力。壳聚糖的修饰改善了机械性能。生物学检测表明 AFSP 和 AFSPC 均无细胞毒性。AFSPC 显示出良好的细胞附着、增殖和迁移能力。总之，改性组织植物可以成为软、硬组织工程的良好候选材料。
