There has been a substantial rise in the number of vancomycin-resistant (VRSA) strains during the last several years. The proportion of vancomycin-resistant strains among isolated has risen steadily in recent years, with the first spike occurring in critical care units and thereafter in general hospital wards. isolates from urinary tract infection patients were studied for their prevalence and antibiotic resistance. From 292 urine samples, 103 bacterial strains (35.3%) were identified as . Various antibiotics were used to test the isolates' antibacterial resistance profiles. Antibiotic resistance to erythromycin was found in most bacterial isolates, whereas tobramycin antibiotic sensitivity was found in most of them. Vancomycin resistance was found in 23 of all isolates in this study. Analysis for β-lactamase found that 71% of isolates were positive in all isolates. There was a single plasmid with a molecular weight of 39.306 Kbp in five selected VRSA isolates that was subjected to plasmid analysis. There was evidence of vancomycin resistance among the isolates collected from UTI patients in this investigation. This vancomycin resistance pretenses a challenge in the treatment of infections and the need to precisely recognize persons who require last-resort medication such as tobramycin.