Parihar Jagdish, Parihar Suraj P, Suravajhala Prashanth, Bagaria Ashima
Department of Physics, Manipal University Jaipur, Jaipur 303007, India.
Wellcome Centre for Infectious Diseases Research in Africa (CIDRI-Africa), Institute of Infectious Diseases and Molecular Medicine (IDM), Division of Medical Microbiology, Faculty of Health Sciences, University of Cape Town, Private Bag X3, Rondebosch, Cape Town 7701, South Africa.
Biology (Basel). 2022 Mar 17;11(3):461. doi: 10.3390/biology11030461.
The arid and semi-arid regions of Rajasthan are one of the most extreme biomes of India, possessing diverse microbial communities that exhibit immense biotechnological potential for industries. Herein, we sampled study sites from arid and semi-arid regions of Thar Desert, Rajasthan, India and subjected them to chemical, physical and metagenomics analysis. The microbial diversity was studied using V3-V4 amplicon sequencing of 16S rRNA gene by Illumina MiSeq. Our metagenomic analyses revealed that the sampled sites consist mainly of Proteobacteria (19-31%) followed by unclassified bacteria (5-21%), Actinobacteria (3-25%), Planctomycetes (5-13%), Chloroflexi (2-14%), Bacteroidetes (3-12%), Firmicutes (3-7%), Acidobacteria (1-4%) and Patescibacteria (1-4%). We have found Proteobacteria in abundance which is associated with a range of activities involved in biogeochemical cycles such as carbon, nitrogen, and sulphur. Our study is perhaps the first of its kind to explore soil bacteria from arid and semi-arid regions of Rajasthan, India. We believe that the new microbial candidates found can be further explored for various industrial and biotechnological applications.
拉贾斯坦邦的干旱和半干旱地区是印度最极端的生物群落之一，拥有多样的微生物群落，这些群落对工业展现出巨大的生物技术潜力。在此，我们从印度拉贾斯坦邦塔尔沙漠的干旱和半干旱地区采集了研究地点的样本，并对其进行化学、物理和宏基因组学分析。使用Illumina MiSeq对16S rRNA基因的V3-V4扩增子进行测序，研究微生物多样性。我们的宏基因组分析表明，采样地点主要由变形菌门（19%-31%）组成，其次是未分类细菌（5%-21%）、放线菌门（3%-25%）、浮霉菌门（5%-13%）、绿弯菌门（2%-14%）、拟杆菌门（3%-12%）、厚壁菌门（3%-7%）、酸杆菌门（1%-4%）和Patescibacteria（1%-4%）。我们发现变形菌门数量丰富，它与一系列参与碳、氮和硫等生物地球化学循环的活动有关。我们的研究可能是首次探索印度拉贾斯坦邦干旱和半干旱地区土壤细菌的此类研究。我们相信，新发现的微生物候选菌株可进一步用于各种工业和生物技术应用的探索。
