Integrative Model-Based Neuroscience Research Unit, University of Amsterdam, Amsterdam, Netherlands.
Department of Neurophysics, Max Planck Institute for Human Cognitive and Brain Sciences, Leipzig, Germany.
Sci Adv. 2022 Apr 29;8(17):eabj7892. doi: 10.1126/sciadv.abj7892. Epub 2022 Apr 27.
We present the first three-dimensional (3D) concordance maps of cyto- and fiber architecture of the human brain, combining histology, immunohistochemistry, and 7-T quantitative magnetic resonance imaging (MRI), in two individual specimens. These 3D maps each integrate data from approximately 800 microscopy sections per brain, showing neuronal and glial cell bodies, nerve fibers, and interneuronal populations, as well as ultrahigh-field quantitative MRI, all coaligned at the 200-μm scale to the stacked blockface images obtained during sectioning. These unprecedented 3D multimodal datasets are shared without any restrictions and provide a unique resource for the joint study of cell and fiber architecture of the brain, detailed anatomical atlasing, or modeling of the microscopic underpinnings of MRI contrasts.
我们呈现了人类大脑细胞和纤维结构的首个三维（3D）一致性图谱，结合了组织学、免疫组织化学和 7-T 定量磁共振成像（MRI），在两个个体样本中。这些 3D 图谱每一个都整合了每个大脑约 800 个显微镜切片的数据，显示神经元和神经胶质细胞体、神经纤维和中间神经元群体，以及超高场定量 MRI，所有数据都在 200-μm 尺度上与切片过程中获得的堆叠块面图像对齐。这些前所未有的 3D 多模态数据集无限制地共享，为大脑细胞和纤维结构的联合研究、详细的解剖图谱或 MRI 对比微观基础的建模提供了独特的资源。
