Harakeh Steve, Saber Saber H, Akefe Isaac O, Shaker Soad, Barkaat Hussain Muhammad, Saad Almasaudi Arwa, Saleh Shaimaa M M, Almasaudi Saad
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Yousef Abdul Latif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
Saudi J Biol Sci. 2022 Apr;29(4):3040-3050. doi: 10.1016/j.sjbs.2022.01.031. Epub 2022 Jan 20.
Recent years have reported a rise in the occurrence of gastric ulceration especially among young children and adults. This study investigated the mechanism by which two types of Saudi honey: Alnahal Aljawal honey (Wadi) or Bin Ghaithan honey (Talh) exerted their antiulcer potential in indomethacin-induced gastric ulceration. Four cohorts of rats were used: Group 1; Healthy controls, Group 2; Ulcerative animals, Group 3; Ulcerative + Wadi honey treatment, Group 4; Ulcerative + Talh honey treatment. We profiled the levels of different indicators of oxidative stress including the activities of gastric mucosal glutathione superoxide dismutase (SOD), catalase (CAT), peroxidase (GPx), reduced glutathione (GSH), and lipid peroxidation (measured as malondialdehyde; MDA). CRP content, IL-10, and plasma tumor necrosis factor-α were also evaluated. The stomach was visually examined for macroscopic lesions and using light microscope for histopathological changes in the glandular mucosa. Wadi or Talh honey significantly reduced the ulcer indices, and essentially protected the glandular mucosa from lesions. Wadi or Talh honey also significantly reduced the gastric mucosal concentrations of GPx, SOD and GSH. In addition, the administration of Wadi or Talh honey decreased gastric mucosal plasma TNF-α and MDA, CRP content, and IL-10 levels. In conclusion, Wadi or Talh honey possibly exerted their antiulcer potential via restoring the homeostasis and stabilizing the enzymatic (SOD and GPx) and non-enzymatic (GSH) antioxidants as well as reducing the levels of inflammatory cytokines (TNF-α, CRP content, IL-10 and, NF-κB activity), and inhibiting the lipid peroxidation in the gastric mucosa. Consequently, Wadi or Talh honey may be of beneficial therapy for patients diagnosed with gastric ulceration. Clinical studies need to be conducted to further support these findings.
近年来，胃溃疡的发生率呈上升趋势，尤其是在幼儿和成年人中。本研究调查了两种沙特蜂蜜：Alnahal Aljawal蜂蜜（瓦迪）或Bin Ghaithan蜂蜜（塔尔）在消炎痛诱导的胃溃疡中发挥抗溃疡潜力的机制。使用了四组大鼠：第1组为健康对照组；第2组为溃疡性动物组；第3组为溃疡性+瓦迪蜂蜜治疗组；第4组为溃疡性+塔尔蜂蜜治疗组。我们分析了氧化应激的不同指标水平，包括胃黏膜谷胱甘肽超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（GPx）、还原型谷胱甘肽（GSH）的活性以及脂质过氧化（以丙二醛；MDA衡量）。还评估了CRP含量、IL-10和血浆肿瘤坏死因子-α。对胃进行肉眼检查以观察宏观病变，并使用光学显微镜观察腺黏膜的组织病理学变化。瓦迪或塔尔蜂蜜显著降低了溃疡指数，并从根本上保护了腺黏膜免受损伤。瓦迪或塔尔蜂蜜还显著降低了胃黏膜中GPx、SOD和GSH的浓度。此外，给予瓦迪或塔尔蜂蜜可降低胃黏膜血浆TNF-α和MDA、CRP含量以及IL-10水平。总之，瓦迪或塔尔蜂蜜可能通过恢复体内平衡、稳定酶促（SOD和GPx）和非酶促（GSH）抗氧化剂以及降低炎症细胞因子（TNF-α、CRP含量、IL-10和NF-κB活性）水平并抑制胃黏膜中的脂质过氧化来发挥其抗溃疡潜力。因此，瓦迪或塔尔蜂蜜可能对诊断为胃溃疡的患者具有有益的治疗作用。需要进行临床研究以进一步支持这些发现。
