Nagam Hanumantharao Samerender, Alinezhadbalalami Nastaran, Kannan Srinivas, Friske Meghan, Rao Smitha
Department of Biomedical Engineering, Michigan Technological University Houghton MI 49931 USA
RSC Adv. 2019 Dec 16;9(69):40190-40195. doi: 10.1039/c9ra07777d. eCollection 2019 Dec 3.
In tissue engineering, the use of scaffolds helps establish a synergistic relationship between the scaffolds and the tissues by improving cell-scaffold interaction. This interaction is enhanced when physiologically relevant biophysical cues are replicated in the artificial scaffolds. Here, we present a novel scaffold that mimics the natural anisotropy of the native extracellular matrix of tissues, fabricated by electrospinning a combination of three polymers: polycaprolactone (PCL), polyvinylidene fluoride (PVDF) and polyaniline (PANI). The scaffolds were characterized for their morphology, surface and mechanical properties. Rat cardiomyoblast (H9c2) cells, cultured on the PCL-PANI-PVDF scaffold, demonstrated cell alignment, penetration and proliferation across the entire surface area of the scaffold without any external chemical or physical stimuli. The PCL-PANI-PVDF scaffold, unlike other scaffolds, does not require post-processing or specific temperature conditions of storage, prior to use. These acellular scaffolds fabricated through polymer blending, open new avenues for research on functional acellular scaffolds for tissue engineering, based on synthetic materials.
在组织工程中，支架的使用通过改善细胞与支架的相互作用，有助于在支架与组织之间建立协同关系。当在人工支架中复制生理相关的生物物理线索时，这种相互作用会增强。在此，我们展示了一种新型支架，它模仿组织天然细胞外基质的自然各向异性，通过静电纺丝三种聚合物的组合制成：聚己内酯（PCL）、聚偏二氟乙烯（PVDF）和聚苯胺（PANI）。对这些支架的形态、表面和力学性能进行了表征。在PCL-PANI-PVDF支架上培养的大鼠心肌母细胞（H9c2），在没有任何外部化学或物理刺激的情况下，在支架的整个表面积上表现出细胞排列、渗透和增殖。与其他支架不同，PCL-PANI-PVDF支架在使用前不需要后处理或特定的储存温度条件。这些通过聚合物共混制备的无细胞支架，为基于合成材料的组织工程功能性无细胞支架研究开辟了新途径。
