全基因组鉴定和基因家族及其对干旱胁迫的转录响应。

Genome-Wide Identification of the Gene Family in and Their Transcriptional Responses to Drought Stress.

机构信息

College of Forestry, Northwest Agriculture and Forestry University, Yangling, Xianyang 712100, China.

Research Centre for Engineering and Technology of Zanthoxylum State Forestry Administration, Yangling, Xianyang 712100, China.

出版信息

Int J Mol Sci. 2022 Apr 26;23(9):4769. doi: 10.3390/ijms23094769.

NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) are one of the largest plant-specific TF families and play a pivotal role in adaptation to abiotic stresses. The genome-wide analysis of NAC TFs is still absent in . Here, 109 ZbNAC proteins were identified from the genome and were classified into four groups with NAC proteins. The 109 genes were unevenly distributed on 46 chromosomes and included 4 tandem duplication events and 17 segmental duplication events. Synteny analysis of six species pairs revealed the closely phylogenetic relationship between and . . Twenty-four types of -elements were identified in the promoters and were classified into three types: abiotic stress, plant growth and development, and response to phytohormones. Co-expression network analysis of the s revealed 10 hub genes, and their expression levels were validated by real-time quantitative polymerase chain reaction (qRT-PCR). Finally, , , , , and were considered the pivotal NAC genes for drought tolerance in . This study represented the first genome-wide analysis of the NAC family in , improving our understanding of NAC proteins and providing useful information for molecular breeding of .

NAC（NAM、ATAF1/2 和 CUC2）转录因子（TFs）是最大的植物特异性 TF 家族之一，在适应非生物胁迫方面发挥着关键作用。在中，仍然缺乏对 NAC TF 的全基因组分析。在这里，从基因组中鉴定出 109 个 ZbNAC 蛋白，并将它们分为四个组，其中包含 55 个 NAC 蛋白。这 109 个基因不均匀地分布在 46 条染色体上，包括 4 个串联重复事件和 17 个片段重复事件。六个物种对的共线性分析表明与之间存在密切的系统发育关系。在启动子中鉴定出 24 种 - 元件，分为三类：非生物胁迫、植物生长发育和对植物激素的反应。对 s 的共表达网络分析显示了 10 个枢纽基因，通过实时定量聚合酶链反应（qRT-PCR）验证了它们的表达水平。最后，、、、和被认为是中耐旱性的关键 NAC 基因。这项研究代表了对中 NAC 家族的首次全基因组分析，提高了我们对 NAC 蛋白的理解，并为的分子育种提供了有用的信息。